Technique / Molecular Biology / Transfection and infection / Transient transfection

Transient transfection is a technique to transiently introduce foreign DNA/RNA into eukaryotic cells through transient opening of cell plasma membrane and subsequent uptake of material. Supercoiled plasmid DNA, siRNA, antibodies, may be transfected using transfection reagent such as cationic lipid.

OBJECTIVE: To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells. METHODS: After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and untransfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM). RESULTS: The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased. CONCLUSIONS: SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.