Molecular Biology / PCR / 5' and 3' RACE protocols and methods

Technique / Molecular Biology / PCR / 5' and 3' RACE

Rapid amplification of cDNA end on 5' or 3' end.

3' RACE PCR new protocol
3' RACE PCR Protocol: 1. First strand cDNA synthesis: - Reaction set up (total volume 20 ul) cDNA synthesis buffer 4 ul dNTP mixture 2 ul oligo-dT anchor primer 1 ul total RNA ...

5' RACE PCR new protocol
5' RACE PCR Protocol 1. First strand cDNA synthesis: - Reaction set up: cDNA synthesis buffer 4 ul dNTP mixture 2 ul gene specific primer 1 1 ul total RNA ...

RACE amplification protocol protocol
A detailed protocol on performing 5' and 3' RACE PCR. (Hiroyuki Takeda lab, National institute of Genetics). ...

cDNA Synthesis with Oligo dT Primers: First strand synthesis protocol
A pdf protocol from Lahn lab at University of Chicago. This may be done with total RNA or poly-A RNA prepped from cell. Here one copy of cDNA will be made with a poly-T primer. (May isolate total RNA and then prep mRNA by selection with oligoT beads to bi ...

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06-Jan-2009 10:51 am

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Technique / Molecular Biology / PCR / 5' and 3' RACERapid amplification of cDNA end on 5' or 3' end.

Technique / Molecular Biology / PCR / 5' and 3' RACE
Rapid amplification of cDNA end on 5' or 3' end.