Protein extract concentrationSummary: Concentrate protein solution into small volume, its introduction and related products.
Content: In protein biochemistry study, it is important to concentrate the initial protein extract into small volume.
Methods for concentrate protein extract into small volume:
- Freeze drying: this method removes water by sublimation.
- Dialysis: this method utilizes the semi-permeable membrane to exchange small molecules between two solutions with different concentration. It is commonly used to desalt the protein solution and for buffer exchange.
- Ultrafiltration: It can be used for efficient buffer exchange and protein desalting.
- Protein precipitation by PEG (polyethylene glycol)
- Protein precipitation by ethanol and acetone.
Keywords: protein concentration
Last update: 2007-02-09 17:06:42 |
