DNA dephosphorylation

Summary: DNA dephosphorylation

Content: Vector dephosphorylation step

Dephosphorylation step is necessary to prevent self-ligation of cloning vectors in blunt-end and single restriction enzyme cloning. Calf intestinal alkaline phosphatase (CIAP) is most frequently used to remove 5' phosphate groups from cloning vector. To calculate the amount pmol of ends for linear dsDNA, use equation bellow.

(ug DNA/(kb size of DNA))*3.04

Keywords: DNA dephosphorylation
Last update: 2007-03-09 16:07:44