PCR primer design

Summary: Introduction and online resources on PCR primer design.

Content: Online PCR primer design tools

• Primer 3

The famouse primer design program designed by Steve Rozen and Whitehead Institute for Biomedical Research.


• 
  CODEHOP
  

Introduction: The CODEHOP program designs PCR (Polymerase Chain Reaction) primers from protein multiple-sequence alignments. The program is intended for cases where the protein sequences are distant from each other and degenerate primers are needed.


• Gene Fisher

Introduction: This online PCR primer design program processes aligned or unaligned amino acid or nucleotide sequences. It can also be used for degenerate primer design.


• Doprimer

Introduction: Doprimer primer design tool can be used for applications targeted on whole genomes or parts of genomes.


• Web Primer

This program can design PCR and DNA sequencing primer.


• PCR Designer for Restriction Analysis of Sequence Mutations

This primer design tool is for restriction analysis of polymorphisms (SNPs).

Reference:

Bioinformatic tools and guideline for PCR primer design (PDF file) (By Kamel A. Abd-Elsalam)

Keywords: PCR primer design
Last update: 2006-09-13 20:47:13