Multiplex PCR optimization and technique papersSummary: Introduction of multiplex PCR, its reaction optimization and some technical papers.
Content: Multiplex PCR amplifies multiple DNA targets using more than one pair of primers in one reaction tube. Multiplex PCR has been successfully applied on genetics screening, genotyping, diagnositic analysis of infectious disease etc. It is also cost-saving for large scale PCR analysis. Quantitative multiplex PCR requires extensive multiplex PCR reaction optimization to determine the primer concentration, DNA template concentration, PCR cycling condition and PCR buffer components.
How to perform multiplex PCR optimization?
Multiplex PCR technique papers:
Garcia-Garcia AB, Blesa S, Martinez-Hervas S, Mansego ML, Gonzalez-Albert V, Ascaso JF, Carmena R, Real JT, Chaves FJ. Semiquantitative multiplex PCR: a useful tool for large rearrangement screening and characterization. Hum Mutat. 2006 Aug;27(8):822-8.
Beigel R., A.N., Apaydin S.M., Fortnow L., and Kasif S. 2001. An Optimal Multiplex PCR Protocol for Closing Gaps in Whole Genomes (RECOMB 01).
Tettelin, H., Radune, D., Kasif, S., Khouri, H. & Salzberg, S.L. 1999. Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project. Genomics 62,500-7.
Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS. 2001. Real-time multiplex PCR assays. Methods. 25(4):430-42.
Markoulatos P, Siafakas N, Katsorchis T, Moncany M. Multiplex PCR: rapid DNA cycling in a conventional thermal cycler. J Clin Lab Anal. 2003;17(4):108-12.
Keywords: multiplex pcr, optimization
Last update: 2006-09-13 16:43:22 |
