Paraffin-Embedded Tissues PCRSummary: Paraffin-Embedded Tissues PCR introduction and related products.
Content: Paraffin-Embedded Tissues (PET) can be processed for genomic DNA extraction and PCR amplification. Paraffin-Embedded Tissues PCR has been applied on analysis of loss of heterozygosity, gene characterization etc. Paraffin-Embedded Tissues normally contains degraded short DNA fragments that is not normally ideal for PCR amplification. Two steps for Paraffin-Embedded Tissues PCR: 1. Purification of DNA from Paraffin-Embedded Tissues (using several commercially available kits, such as QIAquick™ gel extraction kit). 2. Amplify DNA with high quality Taq polymerase (AmpliTaq).
Keywords: Paraffin-Embedded Tissues PCR
Last update: 2007-02-09 02:15:17 |
