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Products overview

Superior Purity

Ultra High Yield

Time Saving

Environment Friendly

Low Cost.

Plasmid DNA purification kits (mini, midi, maxi, and mega) with column and 96 well plate format. HP plasmid kits for plasmid DNA purification from endA+ E.Coli. Magnetic beads plasmid DNA kit.

Virus purification kits for adenovirus, adeno-associated virus (AAV), retrovirus, lentivirus, and HCV virus purification.

High efficiency transfection reagents for variety of cell lines and primary culture transfection.

Genomic DNA and RNA extraction / isolation kits with different size and format (column and 96 well plate format) to isolation DNA/RNA from plant, forensic sample, blood, and cell/tissue cultures.

Our customers currently include researchers from Princeton University, Columbia University, CalTech, Eli Lilly, Stanford University, SUNY Stony Brook, Texas Tech Univ health science center, Dow Pharmaceutical Sciences, McGill University, NCI etc.

GeneTran Transfection Reagents

 

Introduction:

 

GeneTran is a lipid-based reagent designed and formulated with proprietary technology for transfecting DNA  or  siRNA  into  a  variety  of  eukaryotic  cell lines, including HEK293, CHO, COS, NIH3T3, MCF-7, PC12, RKO, C2C12 myoblasts,  mouse  embryonic  stem  cells  (ES)  and  fibroblasts  (MEF), mesenchymal  stem  cells, NCCIT, S2  (Schneider) Drosophila  cells as well as  primary  cultures  of  human  carcinoma,  rat  adrenal  chromaffin  cells, mouse and rat cortical neurons, hepatocytes, and etc. GeneTran also offers extremely high  transfection  efficiencies  and  minimal  cytotoxicity  for suspension  cultures  including  HEK  293,  mouse  lymphocytes,  and  insect cells  (sf9,  sf21,  High-Five). The  reagent  works  very  well  in  cultures  with serum concentration range from 0 to 20% FBS. 

 

 

Exceptional efficiency of transfection for a broad range of adherent cell types.

Especially efficient for embryonic stem (ES) cells and difficult-to-transect primary cultures.

Equally efficient for suspension cultures of HEK293, and sf9, sf21 and High 5 insect cells.

Higher efficiency and lower toxicity than all commercially available transfection reagents currently on the market.

Transfection efficiency not affected by serum. Works equally well in serum-containing (up to 20% FBS) or serum-free media.

Non-toxic or minimal cytotoxicity.

Extremely simple protocol for transfection, using only one reagent without the need for diluents or enhancers.

High levels of recombinant protein production at 24-72 hours after transfection.

Works very well for both single DNA transfection and multi-DNA co-transfection.

Low cost (Almost 50% price off compare to other major commercial products).

 

EZgene Plasmid DNA Purification System

 

Introduction:

 

One of the keys to the EZgeneTM kit is our proprietary buffer system which allows for DNA to be bound to the matrix while unwanted proteins and other contaminates were removed. Nucleic acids are easily eluted with de-ionized water or TE buffer. With patents held, our plasmid DNA purification system could achieve high yield without the presence of chaotropic salt, which is baned to use in gene therapy by FDA. The typical range of OD260 /280 purified DNA utilizing EZgeneTM is normally within 1.75 – 1.85.

 

 

Features:

 

  • High purity : 

No chemicals that may cause protein degeneration;
No Guanidine-Hcl, CsCl, inon exchange resin, and EB;
Enough for multiple applications.

  • High yield: 

1 ~ 5ml bacterial suspension yield 10-30 μg plasmid DNA;
100 ~ 200 mL bacterial suspension yield 1 mg plasmid DNA;   

  • Time-saving: 

For centrifuge method, only 60 min is needed for Midi and Maxi;
For filtration, only 45 min is needed for Midi and Maxi;
Filtration method for Mega kit, only 60 min is needed.

  • Flexibility: 

Choice of spin column or filtration system;
Choice of centrifuge or vacuum purification formats.

  • Lower cost.
 
  • No toxic chemicals in the solution, which is safer for people;
    No toxic chemicals in the waste, which is easy to dispose and good for environment.

 

ViraTrap Virus Purification System

 

Introduction:

 

ViraTrap™ is the proprietary virus purification system, which provides fast and reliable methods to purify virus particles in 60-120 minutes while the traditional CsCl ultracentrifugation takes 24 hours. Only five company owns comparative technology all around the world. Our spin column technology to purify virus is fast, stable, unique and world leading.

 

Features:

 

Fast and reliable

only 60-120 min is needed for purifying

High capacity

 

 

 

Table 1. The column capacity  of ViraTrap™ virus purification system

Product name

Capacity

ViraTrapTM adenovirus associate virus purification kit

1 x 1013 VPs

ViraTrapTM adenovirus purification Kit

3 x 1012 VPs

ViraTrapTM lentivirus purification kit

3 x 1012 VPs

ViraTrapTM retrovirus purification kit

3 x 1012 VPs

ViraTrapTM hepatitis C purification kit

3 x 1012 VPs

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