Books on 'methods-in-molecular-biology'
Total books: 1356 Page 9 of 136
publisher: Humana Press, published: 1996-06-15
ISBN: 0896033368
sales rank: 3111408
Product Description
University of East London, U.K. Manual on laboratory methods on protein isolation for students or researchers new to the technique. Includes a chapter on chromatography. Plastic comb spiral binding. 33 contributors, 3 U.S.
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publisher: Humana Press, published: 2005-11
ISBN: 1592599974
Product Description
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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publisher: Humana Press, published: 2005-12-05
ISBN: 1588293793
sales rank: 1628465
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A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.
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publisher: Humana Press, published: 2010-08-01
ISBN: 1607617528
sales rank: 1288355
Product Description
Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.
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publisher: Humana Press, published: 2008-10-23
ISBN: 1934115800
sales rank: 1208118
Product Description
While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology? series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.
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publisher: Humana Press, published: 2008-10-20
ISBN: 1588294315
sales rank: 1644386
Product Description
In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition, presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.
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publisher: Humana Press, published: 2004-01-09
ISBN: 1588292363
sales rank: 1297937
Product Description
Univ. of South Carolina, Columbia. Provides state-of-the-art techniques for studying genetic recombination in eukaryotes. Also presents methods for using recombination as a tool for producing targeted genetic modification. Features step-by-step instructions for each procedure. For researchers. DNLM: Recombination, Genetic.
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publisher: Humana Press, published: 2009-06-29
ISBN: 160327992X
sales rank: 1279105
Product Description
With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
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publisher: Humana Press, published: 2006-09-15
ISBN: 1588295532
sales rank: 1418496
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A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biology? series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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publisher: Humana Press, published: 2001-01-15
ISBN: 0896037169
sales rank: 4580075
Product Description
Belfast City Hospital Trust, Northern Ireland. Provides detailed practical procedures for a range of the most commonly applied DNA sequencing methods. Strives to pinpoint those areas within each of the techniques that cause the most problems. Previous edition: c1993. Hardcover, plastic-comb binding also available.
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methods in molecular biology, PCR, RT-PCR and real-time quantitative PCR, Differential Display, recombinant DNA, gene therapy, virus protocols, lentivirus methods, gene targeting, mouse knock-out and knock-in, transgenic technology, phenotyping, gene delivery and transfer, transcriptional regulation, RNA methods, RNA Polymerase, gene expression, protein translation regulation, protein kinase, protein phosphorylation, genomics, genomics methods, epigenetics, DNA methylation, DNA sequencing, RNA interference, microarray
Total 1356 books of 136 pages