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by: Mark T. Quinn
publisher: Humana Press, published: 2007-08-02
ASIN: 1588297888
EAN: 9781588297884
sales rank: 687263
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
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publisher: Humana Press, published: 2001-06-01
ASIN: 0896038017
EAN: 9780896038011
sales rank: 575292
Univ. of Hertfordshire, Hatfield, UK. Treasury of all the key molecular biology methods, ranging from DNA extraction to gene localization in situ, needed to function effectively in the modern laboratory. Provides scientific basis of each technique, and a listing of all materials needed, and instructions. For researchers. Hardcover, softcover also available.
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publisher: Humana Press, published: 2008-04-16
ASIN: 1588298280
EAN: 9781588298287
sales rank: 489855
In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.
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by: Alex Dopico
publisher: Humana Press, published: 2007-08-30
ASIN: 1588296628
EAN: 9781588296627
sales rank: 2280390
Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.
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publisher: Humana Press, published: 2008-01-18
ASIN: 158829837X
EAN: 9781588298379
sales rank: 586483
The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. In Clinical Proteomics, a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry and protein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful Methods in Molecular Biology™ series. With its multi-disciplinary approach, Clinical Proteomics provides integrated, cutting-edge techniques sure to be invaluable to a wide range of researchers including clinicians, molecular biologists, chemists, bioinformaticians and computational biologists.
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publisher: Humana Press, published: 1998-01-05
ASIN: 0896034976
EAN: 9780896034976
sales rank: 6487045
Univ. of Maryland, MD. State-of-the-art for the molecular diagnosis of many specific diseases including: cancer; genetic syndromes; and infectious diseases in humans, plants and animals. Modified-outline format. Halftone illustrations. DNLM: Polymerase Chain Reaction--methods.
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publisher: Humana Press, published: 2009-07-21
ASIN: 1934115401
EAN: 9781934115404
sales rank: 1010332
In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.
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publisher: Humana Press, published: 2008-10-23
ASIN: 1588298868
EAN: 9781588298867
sales rank: 784135
In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. Written in the highly successful Methods in Molecular Biology™ series format, the chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Host-Pathogen Interactions: Methods and Protocols serves as an easy entry point for all those investigating the factors responsible for the pathogenicity of microorganisms.
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publisher: Humana Press, published: 1998-06-15
ASIN: 0896034682
EAN: 9780896034686
sales rank: 7238132
Univ. of Rochester Cancer Center, NY. Text providing a "how-to" experimental guide to aid newcomers and seasoned veterans in their research endeavors. Modified-outline format.
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publisher: Humana Press, published: 2000-07-15
ASIN: 089603643X
EAN: 9780896036437
sales rank: 8431467
Univ. College, Cork, Ireland. Provides guidance to the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology. Features detailed techniques and practical procedures.
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