Books on 'methods-in-molecular-biology'
Total books: 1356 Page 2 of 136
by: David A. Kendall, Stephen J. Hill
publisher: Humana Press, published: 1995-04-04
ISBN: 0896032981
sales rank: 1258740
Product Description
University of Nottingham, U.K. Practical manual of experimental protocols in signal transduction, in a step-by-step format. For researchers. Plastic comb spiral binding.
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publisher: Humana Press, published: 2008-12-02
ISBN: 1934115614
sales rank: 1085541
Product Description
Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology? series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.
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publisher: Humana Press, published: 2003-12-12
ISBN: 1588290670
sales rank: 1205839
Product Description
GlaxoSmithKline Pharmaceuticals, Stevenage, UK. Provides key methodologies needed to understand proteins and their functions, including protocols with step-by-step instruction. Covers advances in technology for separations. Previous edition: c1996. For researchers. DNLM: Proteins, isolation & purification--Laboratory Manuals.
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by: Alex Dopico
publisher: Humana Press, published: 2007-08-30
ISBN: 1588296628
sales rank: 1234510
Product Description
Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.
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publisher: Humana Press, published: 2009-11-02
ISBN: 1607613689
sales rank: 675047
Product Description
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology? series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.
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publisher: Humana Press, published: 2004-10-15
ISBN: 1588295451
sales rank: 1339901
Product Description
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).
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publisher: Humana Press, published: 2009-10-02
ISBN: 1607612135
sales rank: 1057246
Product Description
With the dwindling supplies of fossil fuels and growing concerns regarding climate changes due to green house gasses from these fuels, public opinion has swung dramatically towards favoring the development of renewable energy sources. In Biofuels: Methods and Protocols, career-long experts explore a full range of methods for bioenergy covering important topics such as biomass production and delivery to the biorefinery, detailed biochemical characterization, as well as biotechnological techniques for converting plant matter into fuels and chemicals. Time is of the essence in this field, and this volume aims to provide direction and assistance to the growing cadre of researchers endeavoring to develop new sources of bioenergy with a solid, easy-to-use collection of tried-and-true methods which will save time and effort in the field and the laboratory. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to their respective topics, lists of the necessary equipment, materials and reagents, step-by-step, readily reproducible field and laboratory protocols, and notes on troubleshooting and avoiding common pitfalls. Timely and authoritative, Biofuels: Methods and Protocols seeks to help scientists and engineers as they develop and optimize bioenergy technologies needed to drastically change the course of our energy future as soon as possible.
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publisher: Humana Press, published: 2009-04-01
ISBN: 1603278141
sales rank: 1582661
Product Description
Since the discovery of DNA structure and throughout the ensuing ?DNA era?, the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular Biology? series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.
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publisher: Humana Press, published: 2004-03-01
ISBN: 1588293297
sales rank: 1284312
Product Description
Univ. of Leicester, UK. Presents proven methods for studying G-protein-coupled receptors. Includes ligand binding, generation of model cell lines, as well as interaction and activation of G-proteins. Features step-by-step instructions. For researchers. Previous edition: c1997.
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publisher: Humana Press, published: 2005-08-01
ISBN: 1588293998
sales rank: 815257
Product Description
An up-to-date introduction for the uninitiated into the various "flavors" of chemical genomics with a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, emphasizing the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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methods in molecular biology, PCR, RT-PCR and real-time quantitative PCR, Differential Display, recombinant DNA, gene therapy, virus protocols, lentivirus methods, gene targeting, mouse knock-out and knock-in, transgenic technology, phenotyping, gene delivery and transfer, transcriptional regulation, RNA methods, RNA Polymerase, gene expression, protein translation regulation, protein kinase, protein phosphorylation, genomics, genomics methods, epigenetics, DNA methylation, DNA sequencing, RNA interference, microarray
Total 1356 books of 136 pages