Related books on 'stem-cell-methods'
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by: Lawrence S.B. Goldstein publisher: For Dummies, published: 2010-02-02 ASIN: 0470259280 EAN: 9780470259283 sales rank: 230791 The first authoritative yet accessible guide to this controversial topic Stem Cell Research For Dummies offers a balanced, plain-English look at this politically charged topic, cutting away the hype and presenting the facts clearly for you, free from debate. It explains what stem cells are and what they do, the legalities of harvesting them and using them in research, the latest research findings from the U.S. and abroad, and the prospects for medical stem cell therapies in the short and long term.
Comprehensive and unbiased, Stem Cell Research For Dummies is the only guide you need to understand this volatile issue. |
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publisher: Wiley-Blackwell, published: 2011-01-04 ASIN: 0470595450 EAN: 9780470595459 sales rank: 966745 Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features:
The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. |
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publisher: Academic Press, published: 2009-05-06 ASIN: 012375061X EAN: 9780123750617 sales rank: 696106 As part of the Reliable Lab Solutions series, this volume offers an abridged and comprehensive update of selected chapters that first appeared in the three-volume stem cell series published in Methods in Enzymology. Currently, stem cells are of great interest to scientists and clinicians due to their unique ability to differentiate into various tissues of the body, making them a promising source of cells for regenerative medicine and drug discovery as well as an excellent model of vertebrate development. Essential Stem Cell Methods features a detailed set of protocols written by experts in the field and hand-selected by the editors to help researchers drive advances in this fast-moving field. The result is a clear set of step-by-step methods which steer the reader through the challenges of stem cell isolation and differentiation and provide a handy guide for researchers in the field. * Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research * Refines, organizes and updates popular methods from flagship series, Methods in Enzymology *Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid |
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publisher: Humana Press, published: 2010-11-19 ASIN: 161737959X EAN: 9781617379598 sales rank: 1198136 During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs. |
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publisher: Humana Press, published: 2006-02-15 ASIN: 1588294986 EAN: 9781588294982 sales rank: 1774131 Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications. |
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by: Vivi M. Heine publisher: Springer, published: 2011-11-23 ASIN: 9400728158 EAN: 9789400728158 sales rank: 1801625 Brain diseases can have a large impact on patients and society, and treatment is often not available. A new approach in which somatic cells are reprogrammed into induced pluripotent cells (iPS cells) is a significant breakthrough for regenerative medicine. This promises patient-specific tissue for replacement therapies, as well as disease-specific cells for developmental modeling and drug treatment screening. However, this method faces issues of low reprogramming efficiency, and poorly defined criteria for determining the conversion of one cell type to another. Cells contain epigenetic “memories” of what they were that can affect reprogramming. This book discusses the various methods to reprogram cells, the control and determination of cell identity, the epigenetic models that have emerged and the application of iPS cell therapy for brain diseases, in particular Parkinson’s disease and Vanishing White Matter (VWM). |
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publisher: Humana Press, published: 2008-12-19 ASIN: 1588299805 EAN: 9781588299802 sales rank: 2025755 With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In "Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols", leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, "Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols" is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. |
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by: Anthony G. Payne publisher: Basic Health Publications, published: 2006-03-15 ASIN: 159120125X EAN: 9781591201250 sales rank: 485479 We are standing at the threshold of a new and exciting medical era-an era of regeneration, rejuvenation, and renewal in which stems cells will set the stage for healing and in some cases, the restoration of injured, diseased, and debilitated tissues and organ. While stem cell therapy is still in its infancy, the field is rich with promise. The debate over the use of embryonic stem cells and the questionable effectiveness of adult stem cells have led many scientists and clinicians to concentrate their energies on umbilical-cord-derived stem cells from healthy newborn babies. While these cells are technically classifed as "adult stem cells," they appear to have greater restorative and regenerative potential than stem cells derived from adult tissues due to their young age. Human umbilical-cord stem cells (hUCSCs) have demonstrated great efficacy in promoting the healing of many conditions. In the last decade or so, pure cord-blood stem cells have been used by physcians to treat a mulitude of intractable diseases such as progressive multiple sclerosis, amyotrophic lateral sclerosis, certain degenerative eye disorders, stroke, diabetes, and various forms of heart disease. While certainly no cure all, umbilical-cord stem cell therapy appears to be amassing a respectable track record in terms of both safety and clinical utility. In "Umblical Cord Stem Cell Therapy: The Gift of Healing from Health Newborns", Drs. steenblock and Payne share some of the science that underlies stem cell therapy and put a human face on this field with accounts of people who have benefited from hUCSC treatments. And, in providing this information, they encourage readers to take that bold first step into this vast and wondrous medical frontier. |
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publisher: Humana Press, published: 2010-04-22 ASIN: 1607610620 EAN: 9781607610625 sales rank: 2198677 The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology. |
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publisher: Humana Press, published: 2010-11-02 ASIN: 1607619830 EAN: 9781607619833 sales rank: 799030 Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format. |