recombinant-protein books

Related books on 'recombinant-protein'

Return to life science book list
Number of products: 84
Page 1 of 9

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

publisher: Wiley-VCH, published: 2005-02-11
ASIN: 3527310363
EAN: 9783527310364
sales rank: 1821407
While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section—with strains, genetic elements, vectors and special methods, where applicable—as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems—mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example.
With a foreword by Herbert P. Schweizer, Colorado State University, USA:
"As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."

Recombinant Proteins From Plants: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2008-11-26
ASIN: 1588299783
EAN: 9781588299789
sales rank: 2047800

The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.

Recombinant Protein Protocols: Detection and Isolation (Methods in Molecular Biology)

publisher: Humana Press, published: 1997-03-05
ASIN: 089603481X
EAN: 9780896034815
sales rank: 3567873
1st, 1997, Humana Press. Thomas Jefferson University, PA. Comprehensive compendium of step-bystep gene detection and isolation techniques. Companion to: Recombinant Gene Expression Protocols. 67 contributors, 57 U.S.

Recombinant Protein Drugs: Milestones in Drug Therapy

publisher: Birkhäuser Basel, published: 2001-12-15
ASIN: 3764359048
EAN: 9783764359041
sales rank: 5098408
Presents historical, technical, and clinical aspects of recombinant protein drug discovery and development. Describes technical breakthroughs and pioneering discoveries, as well as their translation into products and business. For scientists and graduate students.

FAST AND CHEAP PROTEIN PURIFICATION: Economical alternatives to conventional chromatography-based recombinant protein purification

by: Mahmoud Reza Banki
publisher: VDM Verlag, published: 2009-06-28
ASIN: 3639154436
EAN: 9783639154436
sales rank: 4082779
Two naturally produced in vivo affinity resin substitutes have been combined with a self-cleaving tag to develop two novel affinity-based purification technologies. The first resin alternative is polyhydroxybutyrate (PHB) granules and the second is elastin-like polypeptides (ELP). An engineered tripartite protein fusion is expressed in E. coli and purified through simple centrifugation. The intein self-cleaving tag then releases the target protein with a mild pH shift. The two systems have been successfully used at laboratory scale to purify more than a dozen proteins varying in size, complexity and activity, demonstrating the proof of principle, high purity and yield attainable. Hence, the cost associated with purification of recombinant proteins is reduced significantly to effectively that of just the culture medium. It is expected that this combination of improved economics and simplicity will constitute a significant breakthrough in both large-scale production of purified proteins and enzymes as well as high-throughput proteomics studies.

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparitive View on Host Physiology

publisher: Springer, published: 2001-12-15
ASIN: 0792371372
EAN: 9780792371373
sales rank: 3380972
The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.

Recombinant Proteins from Plants

publisher: Humana Press, published: 2010-05-19
ASIN: 1617370215
EAN: 9781617370212
Univ. of Aberdeen, Scotland. Text providing comprehensive and detailed protocols covering all the latest molecular approaches. For new and established reseachers and biotechnoloists.

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparative View on Host Physiology

publisher: Springer Netherlands, published: 2009-12-28
ASIN: 9048157560
EAN: 9789048157563

The Recombinant Protein Handbook: Principles and Methods.

by: Pharmacia Fine Chemicals
publisher: Uppsala: Pharmacia Biotech undated., published: 1950-01-01
ASIN: B003AG9C8M

Biotechnology, Recombinant Proteins, Monoclonal Antibodies, Therapeutic Genes, Genomics and Bioinformatics (Biotechnology: A Multi-Volume Comprehensive ... 2nd Completely Rev. Ed.) (Volumes 5a and 5b)

by: Christoph W. Sensen
publisher: Wiley-VCH, published: 2002-01-03
ASIN: 3527304320
EAN: 9783527304325
sales rank: 7193069
In this treatise in eight volumes, volume 1 and 2 explain the basic concepts of microbial science and bioengineering. The 3rd and 4th volumes describe the industrially important products of microbial metabolism, as well as the production of biomass. The 5th volume deals with the production of foods and feeds with the aid of microbes. Volumes 6A and 6B cover microbial transformations and special microbial processes. Volume 8 deals with biodegradations. The series is intended for microbiologists, biochemists, bioengineers, chemical engineers, and food and pharmaceutical chemists working in industry, academia, and the public sector. A subscription price is available for those signing up for the whole series.

Page: 1 2 > 9

Language for page:
Which server:
Operation:
Product category:
Search parameter:
Search Books:

	Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems publisher: Wiley-VCH, published: 2005-02-11 ASIN: 3527310363 EAN: 9783527310364 sales rank: 1821407 While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section—with strains, genetic elements, vectors and special methods, where applicable—as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems—mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
	Recombinant Proteins From Plants: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2008-11-26 ASIN: 1588299783 EAN: 9781588299789 sales rank: 2047800 The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.
	Recombinant Protein Protocols: Detection and Isolation (Methods in Molecular Biology) publisher: Humana Press, published: 1997-03-05 ASIN: 089603481X EAN: 9780896034815 sales rank: 3567873 1st, 1997, Humana Press. Thomas Jefferson University, PA. Comprehensive compendium of step-bystep gene detection and isolation techniques. Companion to: Recombinant Gene Expression Protocols. 67 contributors, 57 U.S.
	Recombinant Protein Drugs: Milestones in Drug Therapy publisher: Birkhäuser Basel, published: 2001-12-15 ASIN: 3764359048 EAN: 9783764359041 sales rank: 5098408 Presents historical, technical, and clinical aspects of recombinant protein drug discovery and development. Describes technical breakthroughs and pioneering discoveries, as well as their translation into products and business. For scientists and graduate students.
	FAST AND CHEAP PROTEIN PURIFICATION: Economical alternatives to conventional chromatography-based recombinant protein purification by: Mahmoud Reza Banki publisher: VDM Verlag, published: 2009-06-28 ASIN: 3639154436 EAN: 9783639154436 sales rank: 4082779 Two naturally produced in vivo affinity resin substitutes have been combined with a self-cleaving tag to develop two novel affinity-based purification technologies. The first resin alternative is polyhydroxybutyrate (PHB) granules and the second is elastin-like polypeptides (ELP). An engineered tripartite protein fusion is expressed in E. coli and purified through simple centrifugation. The intein self-cleaving tag then releases the target protein with a mild pH shift. The two systems have been successfully used at laboratory scale to purify more than a dozen proteins varying in size, complexity and activity, demonstrating the proof of principle, high purity and yield attainable. Hence, the cost associated with purification of recombinant proteins is reduced significantly to effectively that of just the culture medium. It is expected that this combination of improved economics and simplicity will constitute a significant breakthrough in both large-scale production of purified proteins and enzymes as well as high-throughput proteomics studies.
	Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparitive View on Host Physiology publisher: Springer, published: 2001-12-15 ASIN: 0792371372 EAN: 9780792371373 sales rank: 3380972 The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.
	Recombinant Proteins from Plants publisher: Humana Press, published: 2010-05-19 ASIN: 1617370215 EAN: 9781617370212 Univ. of Aberdeen, Scotland. Text providing comprehensive and detailed protocols covering all the latest molecular approaches. For new and established reseachers and biotechnoloists.
	Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparative View on Host Physiology publisher: Springer Netherlands, published: 2009-12-28 ASIN: 9048157560 EAN: 9789048157563
	The Recombinant Protein Handbook: Principles and Methods. by: Pharmacia Fine Chemicals publisher: Uppsala: Pharmacia Biotech undated., published: 1950-01-01 ASIN: B003AG9C8M
	Biotechnology, Recombinant Proteins, Monoclonal Antibodies, Therapeutic Genes, Genomics and Bioinformatics (Biotechnology: A Multi-Volume Comprehensive ... 2nd Completely Rev. Ed.) (Volumes 5a and 5b) by: Christoph W. Sensen publisher: Wiley-VCH, published: 2002-01-03 ASIN: 3527304320 EAN: 9783527304325 sales rank: 7193069 In this treatise in eight volumes, volume 1 and 2 explain the basic concepts of microbial science and bioengineering. The 3rd and 4th volumes describe the industrially important products of microbial metabolism, as well as the production of biomass. The 5th volume deals with the production of foods and feeds with the aid of microbes. Volumes 6A and 6B cover microbial transformations and special microbial processes. Volume 8 deals with biodegradations. The series is intended for microbiologists, biochemists, bioengineers, chemical engineers, and food and pharmaceutical chemists working in industry, academia, and the public sector. A subscription price is available for those signing up for the whole series.