Books on 'protein-purification'
Total books: 136 Page 1 of 14
by: Robert K. Scopes
publisher: Springer, published: 1993-11-19
ISBN: 0387940723
sales rank: 468267
Product Description
The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions of Protein Purification described it as "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), and "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature).
Review
I find this book, protein purification: principles and practice, is one of the best sources to understand the bases of protein purification techniques. The book describes the mechanisms, chemistry bases, and practical considerations of different protein purification methods. Moreover, the book is written easily for scientists/technicians with background ranging from weak to excellent in biochemistry. If you were new to the field of protein purification, this book will help you to build skills in a very short time since it fills the missing gapes in almost famous protocols by providing very easy background information about each purification procedure. I highly recommend to have it on your book shelf.
In the first chapter of Protein Purification, Scopes notes that more and more biochemical techniques are black boxed, with kits eliminating the need for an understanding of biochemical principles (e.g., "precipitate with Acme Solution B, pour off Solution B, and redissolve precipitant in Solution C"). Scopes notes that with a proper understanding of biochemistry, one can save money on kits and do more purification steps with standard lab materials and reagents. He wrote this book to help biochemistry labs make more informed decisions on protein purification rather than just defaulting on some already familiar kit or protocol.Scopes succeeded in this purpose with flying colors: he hit a rare balance that makes this book fun to read. Each chapter is well-laid out, concetrating on a single separation technique's general practice and the theoretical foundations for that method. The basic ideas and materials are laid out, with clear, well-picked illustrations. I was particularly happy with the mathematical treament Scopes gives: understanding the thermodynamics or physics of a process aids your intuitive understanding and makes your troubleshooting skills much more honed.
Scientists and educators alike will benefit from Robert K. Scopes thorough and up-to-date examination of the techniques behind the science of protein purification. Written with enough simplicity and easy reading for the upper-level undergraduate student, but with all the facts and formulas for hard-core theorists, Protein Purification may well be the most concise and easy to read book of its kind on the textbook market. A must read for those interested in any form of protein science, anyone from the industrial biochemist to the graduate student will benefit from Robert's full description of the most current procedures for separating proteins by size, charge, or physical characteristics. An in-depth discussion of procedures used from the beginning of scientists' knowledge of the individual characteristics of protein charge and size to the most current procedures involving dye ligand chromatography will ensure an overall knowledge of the field. Robert's belief that a ninety percent separation of protein is the minimun standard for any reliable purification procedure is augmented by his insistence that most science can be done well while watching a budget. Definitely a textbook for the educator who has less money on hand than the typical research scientist, I highly recommend this book for undergraduate and graduate level biochemistry courses, as its cost is much less than that generally charged for most college texts.
by: Ian M. Rosenberg
publisher: Birkhäuser Boston, published: 2004-10-21
ISBN: 0817643400
sales rank: 600902
Product Description
This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation.
Review
Very useful reference for any biochemistry lab. Less bulkier than cold spring harbor manuals and certainly packed with a lot of useful information. This book would be useful for the professional as well as the novice. Detailed protocols included and updated from the previous edition. The section of post translational modifications was the most useful for my purposes. This book can take you from cells to purified protein under most circumstances with the minimum of repetition. I recommend this book particularly for graduate students in biochemistry who deal with protein purification as well as technicians.
Practical book with very easy to follow and concise protocols about protein purification If you have to purify and analyze proteins but you re not an expert this is the book for you.
This is a model for all protocol lab manuals. It is clear, precise, and well done. Incorporates the experiences of the authors, which are used to perfect the assays and protocols and minimize insufficient results and data. Troubleshooting tips are useful and original. Truly a necessary benchtop reference for the serious researcher.
publisher: Academic Press, published: 2009-12-03
ISBN: 0123749786
sales rank: 802542
Product Description
The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunologyAssembles chapters on both common and less-common relevant techniquesProvides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Review
by: Richard J. Simpson, Peter D. Adams, Erica A. Golemis
publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01
ISBN: 0879698675
sales rank: 449143
Product Description
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extractsÂ?a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed.
Review
by: Philip L.R. Bonner
publisher: Taylor & Francis, published: 2007-04-11
ISBN: 0415385113
sales rank: 988437
Product Description
Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Review
publisher: Oxford University Press, USA, published: 2001-04-05
ISBN: 0199636737
sales rank: 883356
Product Description
Describes strategies and practical procedures for protein purification from common sources and includes chapters on the purification of inclusion bodies, fusion proteins, and proteins for crystallography. Previous edition was titled, Protein Purification Methods, c1989. Softcover, hardcover also available.
Review
publisher: Humana Press, published: 1996-06-15
ISBN: 0896033368
sales rank: 3020651
Product Description
University of East London, U.K. Manual on laboratory methods on protein isolation for students or researchers new to the technique. Includes a chapter on chromatography. Plastic comb spiral binding. 33 contributors, 3 U.S.
Review
publisher: Wiley-VCH, published: 1998-02-27
ISBN: 0471186260
sales rank: 1152084
Product Description
Praise for the First Edition of Protein Purification: "Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry. "[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters. This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: * Gel filtration and ion exchange chromatography * Affinity and covalent chromatography * Reversed phase and hydrophobic interaction chromatography * Electrophoresis in gels * Immunoelectrophoresis and capillary electrophoresis * Protein mapping by two-dimensional polyacrylamide gel electrophoresis * Protein elution and blotting methods * Large-scale electrophoresis processes.
Review
publisher: Humana Press, published: 2003-05-29
ISBN: 1588291243
sales rank: 800018
Product Description
Villanova Univ., PA. Text explains proven methods and protocols for generating membrane proteins from expression to purification. Provides step-by step techniques for determining the structure of membrane proteins including troubleshooting tips. For researchers.
Review
publisher: Humana Press, published: 2008-10-20
ISBN: 1588298795
sales rank: 801388
Product Description
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology? series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
Review
proteomics, pharmacogenomics, mass spectrometry, electrophoresis, chromatography, HPLC, recombinant proteins, protein expression, protein purification, protein-protein protein-DNA/RNA interaction, phage display, drug discovery, biotechnology, pharmacology books
Total 136 books of 14 pages