Related books on 'protein-purification'
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by: Robert K. Scopes publisher: Springer, published: 1993-11-19 ASIN: 0387940723 EAN: 9780387940724 sales rank: 575349 The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry "well organized and written clearly" --American Scientist "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature
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publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636710 EAN: 9780199636716 sales rank: 697750 Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set) are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy. |
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by: Pauline M. Doran publisher: Academic Press, published: 2012-05-30 ASIN: 012220851X EAN: 9780122208515 sales rank: 449375 This welcome new edition covers bioprocess engineering principles for the reader with a limited engineering background. It explains process analysis from an engineering point of view, using worked examples and problems that relate to biological systems. Application of engineering concepts is illustrated in areas of modern biotechnology such as recombinant protein production, bioremediation, biofuels, drug development, and tissue engineering, as well as microbial fermentation. The main sub-disciplines within the engineering curriculum are all covered; Material and Energy Balances, Transport Processes, Reactions and Reactor Engineering. With new and expanded material, Doran's textbook remains the book of choice for students seeking to move into bioprocess engineering. NEW TO THIS EDITION:
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by: Robert K. Scopes publisher: Springer, published: 2010-12-06 ASIN: 1441928332 EAN: 9781441928337 sales rank: 930094 The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry "well organized and written clearly" --American Scientist "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature
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publisher: Wiley, published: 2011-03-22 ASIN: 0471746614 EAN: 9780471746614 sales rank: 1095703 This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
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publisher: Academic Press, published: 2009-12-03 ASIN: 0123749786 EAN: 9780123749789 sales rank: 1146679 The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today.
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by: Scott M. Wheelwright publisher: Wiley-Interscience, published: 1993-11-04 ASIN: 0471037230 EAN: 9780471037231 sales rank: 1285392 A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617378216 EAN: 9781617378218 sales rank: 1986081 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields. |
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publisher: Academic Press, published: 1994-11-21 ASIN: B003N7NIDI sales rank: 967421 A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Key Features * Provides general guidelines and strategies for isolation of membrane proteins * Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs * Gives special emphasis to new native and denaturing electrophoresis techniques * Explains modifications of techniques used for water-soluble proteins |
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by: Rajni Hatti-Kaul publisher: Dekker, published: 2009-02-19 ASIN: B001TMCFCE sales rank: 988676 No description available |