Related books on 'protein-purification'
Return to life science book listNumber of products: 146
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by: Robert K. Scopes publisher: Springer, published: 1993-11-19 ASIN: 0387940723 EAN: 9780387940724 sales rank: 223673 The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions of Protein Purification described it as "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), and "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature). |
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publisher: Academic Press, published: 2009-12-03 ASIN: 0123749786 EAN: 9780123749789 sales rank: 829179 The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today.
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publisher: Wiley-VCH, published: 1998-02-27 ASIN: 0471186260 EAN: 9780471186267 sales rank: 1297670 Praise for the First Edition of Protein Purification: "Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry. "[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters. This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: * Gel filtration and ion exchange chromatography * Affinity and covalent chromatography * Reversed phase and hydrophobic interaction chromatography * Electrophoresis in gels * Immunoelectrophoresis and capillary electrophoresis * Protein mapping by two-dimensional polyacrylamide gel electrophoresis * Protein elution and blotting methods * Large-scale electrophoresis processes. |
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publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636729 EAN: 9780199636723 sales rank: 1624514 Focuses on unit operations and analytical techniques and includes an overview of purification strategy. Covers initial extraction and clarification techniques, key purification methods with an emphasis on chromatography, and scale-up considerations. Hardcover, softcover also available. |
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by: Daniel M. Bollag publisher: Wiley-Liss, published: 1996-01-15 ASIN: 0471118370 EAN: 9780471118374 sales rank: 134907 Protein Methods Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein Reviews from the first edition "...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time."-Biotechnology and Applied Biochemistry "The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." -Theoretical & Applied Genetics "...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." -FEBS Letters This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization. Chapters include * Preparation for Protein Isolation * Protein Extraction and Solubilization * Protein Concentration Determination * Concentrating Protein Solutions * Gel Electrophoresis Under Denaturing Conditions * Gel Electrophoresis Under ...and feature * reagent items and equipment required for each method * chapter "view-at-a-glance" tabs * illustrations for all gel applications and other methods * complete supplier list * appendices of basic chemical parameters and molecular data * key references * descriptive tables * Safety notes. |
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by: Philip L.R. Bonner publisher: Taylor & Francis, published: 2007-04-11 ASIN: 0415385113 EAN: 9780415385114 sales rank: 98635 Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification. |
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by: Ian M. Rosenberg publisher: Birkhäuser Boston, published: 2004-10-21 ASIN: 0817643400 EAN: 9780817643409 sales rank: 1127494 This comprehensive, practical, and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researches and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation. |
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by: Richard J. Simpson publisher: Cold Spring Harbor Laboratory Press, published: 2008-11-01 ASIN: 0879698675 EAN: 9780879698676 sales rank: 911758 Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts- a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Related Titles from the Publisher Proteins and Proteomics: A Laboratory Manual Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. Proteomics: A CSHL Course Manual Molecular Cloning: A Laboratory Manual, 3rd ed. |
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publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636745 EAN: 9780199636747 sales rank: 3616911 Describes strategies and practical procedures for protein purification from common sources and includes chapters on the purification of inclusion bodies, fusion proteins, and proteins for crystallography. Previous edition was titled, Protein Purification Methods, c1989. Hardcover, softcover also available. |
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by: Charles Hardin publisher: Oxford University Press, USA, published: 2001-03-01 ASIN: 0195132947 EAN: 9780195132946 sales rank: 1113780 On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: · Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques · Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations · Discusses fluorophores, and solvent effects on protein structure · Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature · Includes carefully selected primary source research literature and articles from current vendor literature · Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized · Provides an alphabetized list of common reagents for rapid reference · Offers an extensive index of concepts and terms · Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches |