protein-expression books

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High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617378216
EAN: 9781617378218
sales rank: 1986081
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale

by: Charles Hardin
publisher: Oxford University Press, USA, published: 2001-03-01
ASIN: 0195132947
EAN: 9780195132946
sales rank: 1220788
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features:
ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques
ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations
ï¿½ Discusses fluorophores, and solvent effects on protein structure
ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature
ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature
ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized
ï¿½ Provides an alphabetized list of common reagents for rapid reference
ï¿½ Offers an extensive index of concepts and terms
ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches

Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2005-08-15
ASIN: 1588293904
EAN: 9781588293909
sales rank: 990350
World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Protein Expression in Down Syndrome Brain (Springer Medicine)

publisher: Springer, published: 2002-05-17
ASIN: 3211837329
EAN: 9783211837320
sales rank: 4995733
This book presents latest original research work and current opinions on Down syndrome, focussing on the molecular biology of the Down syndrome brain at the protein level, rather than at nucleic acid level, as it is the proteins that do the work, and there is a long and unpredictable way from RNA to protein. The studies used fetal brains with Down syndrome in which no neurodegeneration was found and characterized proteins by two-dimensional gel electrophoresis and mass spectrometry. The book provides new aspects, such as that neuronal density and neurotransmission is unchanged in early life but synaptosomal structures and dendritic spines are already seriously affected at the early second trimester. The presentation of a series of tentative mechanisms for the development of the Down syndrome neurophenotype provides models for present and future thinking on the neurobiology of the Down syndrome brain.

Protein Expression: A Practical Approach (Practical Approach Series)

publisher: Oxford University Press, USA, published: 1999-07-29
ASIN: 0199636230
EAN: 9780199636235
sales rank: 3629757
Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.

Milk Proteins: From Expression to Food (Food Science and Technology)

publisher: Academic Press, published: 2008-10-09
ASIN: 0123740398
EAN: 9780123740397
sales rank: 2922561
In recent years, there has been a great deal of progress in the understanding and management of milk proteins across the production chain. This book takes a uniquely comprehensive look at those developments and presents them in a one-source overview.

By providing a brief overview of each topic area, and then describing the most important recent advances therein, the "field-to-table" approach of this book provides specialists with new and directly relevant information in their own areas, along with information from complementary research fields, allowing them to contextualize their work within the larger pictures. At the same time it provides generalists with a complete overview and offers insights into topics for more in-depth reading.

Covering areas that are receiving attention from people of many fields -- genomics, functional foods -- and including the latest research and developments in milk-protein phenomenon and interactions, this book will be an ideal resource for professionals and students alike.

*A fresh look at recent developments across the entire production chain -- from animal genetics to nutritional and nutrigenomic needs of the customer
*Up-to-date information from internationally-recognised authors from both academic and commercial resources

Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-10-12
ASIN: 1617793515
EAN: 9781617793516
sales rank: 2526353
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.

Heterologous Expression of Membrane Proteins: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-12-18
ASIN: 1607613433
EAN: 9781607613435
sales rank: 2497992
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.

Cell-Free Protein Expression

by: W. Antoni Kudlicki
publisher: Landes Bioscience, published: 2007-11-30
ASIN: 1587061236
EAN: 9781587061233
sales rank: 3435174

Protein Expression in Animal Cells, Volume 43 (Methods in Cell Biology)

publisher: Academic Press, published: 1994-09-22
ASIN: 0125641443
EAN: 9780125641449
sales rank: 8373143
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.

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	High Throughput Protein Expression and Purification: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617378216 EAN: 9781617378218 sales rank: 1986081 Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
	Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale by: Charles Hardin publisher: Oxford University Press, USA, published: 2001-03-01 ASIN: 0195132947 EAN: 9780195132946 sales rank: 1220788 On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations ï¿½ Discusses fluorophores, and solvent effects on protein structure ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized ï¿½ Provides an alphabetized list of common reagents for rapid reference ï¿½ Offers an extensive index of concepts and terms ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches
	Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2005-08-15 ASIN: 1588293904 EAN: 9781588293909 sales rank: 990350 World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
	Protein Expression in Down Syndrome Brain (Springer Medicine) publisher: Springer, published: 2002-05-17 ASIN: 3211837329 EAN: 9783211837320 sales rank: 4995733 This book presents latest original research work and current opinions on Down syndrome, focussing on the molecular biology of the Down syndrome brain at the protein level, rather than at nucleic acid level, as it is the proteins that do the work, and there is a long and unpredictable way from RNA to protein. The studies used fetal brains with Down syndrome in which no neurodegeneration was found and characterized proteins by two-dimensional gel electrophoresis and mass spectrometry. The book provides new aspects, such as that neuronal density and neurotransmission is unchanged in early life but synaptosomal structures and dendritic spines are already seriously affected at the early second trimester. The presentation of a series of tentative mechanisms for the development of the Down syndrome neurophenotype provides models for present and future thinking on the neurobiology of the Down syndrome brain.
	Protein Expression: A Practical Approach (Practical Approach Series) publisher: Oxford University Press, USA, published: 1999-07-29 ASIN: 0199636230 EAN: 9780199636235 sales rank: 3629757 Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.
	Milk Proteins: From Expression to Food (Food Science and Technology) publisher: Academic Press, published: 2008-10-09 ASIN: 0123740398 EAN: 9780123740397 sales rank: 2922561 In recent years, there has been a great deal of progress in the understanding and management of milk proteins across the production chain. This book takes a uniquely comprehensive look at those developments and presents them in a one-source overview. By providing a brief overview of each topic area, and then describing the most important recent advances therein, the "field-to-table" approach of this book provides specialists with new and directly relevant information in their own areas, along with information from complementary research fields, allowing them to contextualize their work within the larger pictures. At the same time it provides generalists with a complete overview and offers insights into topics for more in-depth reading. Covering areas that are receiving attention from people of many fields -- genomics, functional foods -- and including the latest research and developments in milk-protein phenomenon and interactions, this book will be an ideal resource for professionals and students alike. A fresh look at recent developments across the entire production chain -- from animal genetics to nutritional and nutrigenomic needs of the customer Up-to-date information from internationally-recognised authors from both academic and commercial resources
	Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-10-12 ASIN: 1617793515 EAN: 9781617793516 sales rank: 2526353 Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
	Heterologous Expression of Membrane Proteins: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-12-18 ASIN: 1607613433 EAN: 9781607613435 sales rank: 2497992 Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
	Cell-Free Protein Expression by: W. Antoni Kudlicki publisher: Landes Bioscience, published: 2007-11-30 ASIN: 1587061236 EAN: 9781587061233 sales rank: 3435174
	Protein Expression in Animal Cells, Volume 43 (Methods in Cell Biology) publisher: Academic Press, published: 1994-09-22 ASIN: 0125641443 EAN: 9780125641449 sales rank: 8373143 Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.