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by: Sabrina Chase
publisher: Worlds Away Press, published: 2012-01-14
ASIN: B006XL95CY
sales rank: 58765
SHORT STORY--Deep in the bowels of the Bureau of Substandards, new hire Boris Niels discovers a vending machine with a very strange coin in the return slot. Can he get a refreshing beverage and still keep his job? Will he ever find his way back to his home dimension? What is it with aliens and paperwork? PLUS: A section from the upcoming science fiction book The Long Way Home.
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by: Michael L. Altshuler
publisher: Caister Academic Press, published: 2006-03-01
ASIN: 1904455077
EAN: 9781904455073
sales rank: 480157
This unique polymerase chain reaction (PCR) troubleshooting guide is an essential companion for readers with some experience in PCR. The book discusses the many and varied problems encountered with PCR, together with tips, advice, and procedures to obviate rather than overcome the PCR problems. The advice in PCR Troubleshooting is invaluable.
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by: Paul Rabinow
publisher: University Of Chicago Press, published: 1996-05-15
ASIN: 0226701468
EAN: 9780226701462
sales rank: 901699
Making PCR is the fascinating, behind-the-scenes account of the invention of one of the most significant biotech discoveries in our time—the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a given segment in a short period of time. It makes abundant what was once scarce—the genetic material required for experimentation.
Making PCR explores the culture of biotechnology as it emerged at Certus Corporation during the 1980s and focuses on its distinctive configuration of scientific, technical, social, economic, political, and legal elements, each of which had its own separate trajectory over the preceding decade. The book contains interviews with the remarkable cast of characters who made PCR, including Kary Mullin, the maverick who received the Nobel prize for "discovering" it, as well as the team of young scientists and the company's business leaders.
This book shows how a contingently assembled practice emerged, composed of distinctive subjects, the site where they worked, and the object they invented.
"Paul Rabinow paints a . . . picture of the process of discovery in Making PCR: A Story of Biotechnology [and] teases out every possible detail. . . . Makes for an intriguing read that raises many questions about our understanding of the twisting process of discovery itself."—David Bradley, New Scientist
"Rabinow's book belongs to a burgeoning genre: ethnographic studies of what scientists actually do in the lab. . . . A bold move."—Daniel Zalewski, Lingua Franca
"[Making PCR is] exotic territory, biomedical research, explored. . . . Rabinow describes a dance: the immigration and repatriation of scientists to and from the academic and business worlds."—Nancy Maull, New York Times Book Review
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publisher: Humana Press, published: 2010-11-19
ASIN: 1617377430
EAN: 9781617377433
sales rank: 2290562
At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
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by: Stephen A. Bustin
publisher: International University Line, published: 2004-07
ASIN: 0963681788
EAN: 9780963681782
sales rank: 274444
This book is a comprehensive manual to allow both the novice researcher and the expert to set up and carry out quantitative PCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints, methods, and aims to simulate the researchers into generating, interpreting, and publishing data that are reproducible, reliable, and biologically meaningful
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by: M. J. McPherson
publisher: Taylor & Francis, published: 2006-03-30
ASIN: 0415355478
EAN: 9780415355476
sales rank: 747894
A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
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by: Elizabeth van Pelt-Verkuil
publisher: Springer, published: 2010-11-24
ASIN: 9048175798
EAN: 9789048175796
sales rank: 939551
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
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publisher: Taylor & Francis, published: 2006-06-13
ASIN: 041537734X
EAN: 9780415377348
sales rank: 459993
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
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publisher: Academic Press, published: 1995-06-27
ASIN: 0123991668
EAN: 9780123991669
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!
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publisher: Springer, published: 2004-02-20
ASIN: B000QFALZ8
sales rank: 874927
Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
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