Related books on 'in-situ-hybridization'

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publisher: Humana Press, published: 2005-12-15
ASIN: 1588294021
EAN: 9781588294029
sales rank: 1502002
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.

publisher: Humana Press, published: 2014-09-13
ASIN: 1493914588
EAN: 9781493914586
sales rank: 889824

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.


by: Trude Schwarzacher
publisher: BIOS Scientific Publishers, published: 2000-02
ASIN: 0387915966
EAN: 9783540734420
sales rank: 1337578
In situ hybridization is a powerful technique used to characterize and locate nucleic acid sequences within the cell. These methods of molecular cytogenetics have become increasingly important over the last 10 years, and have had an important impact on many different areas of the life sciences.

Practical in situ Hybridization is aimed at researchers who wish to use efficient and reliable protocols in their work and those who need to check the validity and interpretation of published data. This book presents a reliable, tried-and-tested approach to the methodologies in current use in many laboratories. The book has a user-friendly and open format for easy access to information, comprehensive coverage of many in situ hybridization techniques, full color sections illustrating results clearly, and an extensive guides to troubleshooting

The book is an invaluable guide for all researchers who need to understand the principles and practice of in situ hybridization.


publisher: Humana Press, published: 2010-09-09
ASIN: 1607617889
EAN: 9781607617884
sales rank: 2311533
Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

publisher: Springer, published: 2008-12-05
ASIN: 3540705805
EAN: 9783540705802
sales rank: 3535487
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

by: Gerard Morel
publisher: CRC Press, published: 2000-09-28
ASIN: 0849307031
EAN: 9780849307034
sales rank: 4650238
In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

by: Marie-Francoise Chesselet
publisher: CRC Press, published: 1990-07-03
ASIN: 0849369126
EAN: 9780849369124
sales rank: 5230838
The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.

publisher: Oxford University Press, published: 1999-03-25
ASIN: 019854880X
EAN: 9780198548805
sales rank: 8778071
This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances in the last seven years.

publisher: Wiley-Liss, published: 1999-12-03
ASIN: 0471013455
EAN: 9780471013457
sales rank: 3981507
Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. The final section of the book is devoted to the applications of FISH techniques to genetic analysis. This incorporates radiation biodosimetry in chromosomal aberrations, genetic toxicology, and multiprobe methods for detecting chromosomally defective sperm in humans and mice, plus rapid prenatal diagnosis of chromosomal aneuploidies and detection of fetal trisomy. Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry.

by: K. H. Andy Choo
publisher: Humana Press, published: 1994-09-01
ASIN: 0896032809
EAN: 9780896032804
sales rank: 5031855
Thirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans.

The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text.

Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.
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