Related books on 'hplc'
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by: Lloyd R. Snyder publisher: Wiley-Interscience, published: 1997-03-03 ASIN: 047100703X EAN: 9780471007036 sales rank: 319067 This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. |
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by: Michael W. Dong publisher: Wiley-Interscience, published: 2006-06-12 ASIN: 047172789X EAN: 9780471727897 sales rank: 199070 A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include:
While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. |
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by: George Lunn publisher: Wiley-Interscience, published: 1998-11-12 ASIN: 0471164585 EAN: 9780471164586 sales rank: 4965342 Thousands of derivatization procedures for HPLC and CE-an essential tool for today's analytical chemist. This valuable reference offers fast and convenient access to derivatization reactions for both HPLC and capillary electrophoresis (CE). Covering a wide variety of compounds from pharmaceutical drugs and biological products to industrial contaminants, it is organized first by functional group and then by individual reagents. Techniques for each functional group are described in sufficient detail that the researcher can replicate procedures without reference to the original publications-saving hours of tedious library research. And because detailed procedures for the same reagent are listed together, it is easy to combine features of different methods and tailor them to fit specific individual requirements. Also available on CD-ROM, Handbook of Derivatization Reactions for HPLC contains fully abstracted and evaluated procedures from more than 1,900 papers, with descriptions of hundreds of reagents. A further 3,000 papers are referenced in bibliographies that are clearly annotated to help analysts identify those sources likely to be most useful. This important new resource will be welcomed by chemists working in pharmaceutical, biomedical, and environmental analysis. Also available on CD-ROM System requirements . . . IBM-compatible PC 486 or better and Windows(r) 3.0 or higher, or Macintosh 68030 processor and System 7 or higher * CD-ROM drive and 8 MB RAM minimum * 5 MB free hard disk space minimum, 30 MB recommended for full installation. |
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by: Veronika R. Meyer publisher: Wiley-VCH, published: 2006-05-01 ASIN: 3527313729 EAN: 9783527313723 sales rank: 1118672 Adding 13 examples to this new, third edition, Veronika Meyer now offers solutions for nearly 100 problems. All the examples are accompanied by a concise, instructive text and an informative figure, including essential fundamentals as well as such helpful strategies as equipment tests or quality assurance strategies. A practice-oriented aid to obtaining correct and reliable analytical results. From the reviews of a previous edition: 'This is a giant of a little book on HPLC.' (Analytical Chemistry) |
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by: Lloyd R. Snyder publisher: John Wiley & Sons, published: 1988-12 ASIN: 0471627828 EAN: 9780471627821 sales rank: 1275362 This book shows the chemist who is already familiar with the basics of HPLC how to develop HPLC separation methods for a wide variety of situations. It focuses mainly on reversed-phase separations of small-molecules, but also addresses development of normal-phase and ion-pair separations. The authors introduce the use of computers to speed and improve method development, covering commercially available HPLC programs. |
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by: Harald Pasch publisher: Springer, published: 1999-05-14 ASIN: 3540655514 EAN: 9783540655510 sales rank: 858006 Polymers are mainly characterized by molar mass, chemical composition, functionality and architecture. The determination of the complex structure of polymers by chromatographic and spectroscopic methods is one of the major concerns of polymer analysis and characterization. This lab manual describes the experimental approach to the chromatographic analysis of polymers. Different chromatographic methods, their theoretical background, equipment, experimental procedures and applications are discussed. The book will enable polymer chemists, physicists and material scientists as well as students of macromolecular and analytical science to optimize chromatographic conditions for a specific separation problem. Special emphasis is given to the description of applications for homo- and copolymers and polymer blends. |
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by: M. Guo publisher: Elsevier, published: 2006-01-18 ASIN: B000PBZYSM This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: Interaction of traditional Chinese Herb Rhizoma Chuanxiong and protein was studied by microdialysis coupled with high performance liquid chromatography. Compounds in Rhizoma Chuanxiong, such as ferulic acid, senkyunolide A and 3-butylphthalide, were identified by HPLC, HPLC-MS and UV-vis. Microdialysis recoveries and binding degrees of compounds in Rhizoma Chuanxiong with human serum albumin (HSA) and other human plasma protein were determined: recoveries of microdialysis sampling ranged from 36.7 to 98.4% with R.S.D. below 3.1%; while binding to HSA ranged from 0 to 91.5% (0.3mM HSA) and from 0 to 93.5% (0.6mM HSA), respectively. Compared with HSA, most of compounds bound to human blood serum more extensively and the results showed that binding of these compounds in Rhizoma Chuanxiong was influenced by pH. Two compounds were found to bind to HSA and human blood serum, their binding degrees were consistent with ferulic acid and 3-butylphthalide, the active compounds in Rhizoma Chuangxiong. |
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by: Uwe D. Neue publisher: Wiley-VCH, published: 1997-07-28 ASIN: 0471190373 EAN: 9780471190370 sales rank: 1431289 An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: * Comprehensive overview of state-of-the-art HPLC column technology * Explanation of the underlying principles of HPLC columns * Methods for selecting columns * Practical advice on using and applying columns, including examples * Section by M. Zoubair El Fallah on methods development * Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed * Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses. |
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publisher: CRC Press, published: 2010-07-14 ASIN: 1574445545 EAN: 9781574445541 sales rank: 1572485 High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the publication of the benchmark first edition twelve years ago. It reports detailed information on fundamental and practical aspects of HPLC related to conventional format and sophisticated novel approaches which have been developed to address a variety of separation problems in different fields. The latest concepts New concepts presented in this edition include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, and capillary electromigration techniques. The book also discusses LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, field-flow fractionation, retention models for ions, and polymer HPLC. Fundamentals and applications The first section of the book explores emerging novel aspects of HPLC and related separation methods based on the differential velocity of analytes in a liquid medium under the action of either an electric field (capillary electromigration techniques) or a gravitational field (field-flow fractionation). The section focusing on applications highlights four significant areas in which HPLC is successfully employed: chiral pharmaceutical, environmental analysis, food analysis, and forensic science. Ideal for a widespread audience Explanatory figures and tables supplement the text and clarify difficult concepts. Extensive references provide gateways to more focused study. Suitable for undergraduates or new practitioners interested in improving their knowledge on the current status and future trends of HPLC, the book is also a critical resource for researchers looking for solutions to complex separation problems or those who currently use HPLC either as an analytical or a preparative scale tool. |
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by: Marvin McMaster publisher: Wiley-Interscience, published: 2006-12-18 ASIN: 0471754013 EAN: 9780471754015 sales rank: 1207520 This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete with a CD-ROM and appendices, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file. |