The second edition of this volume reflects the recent advances in the FCM analysis of hematopoietic disorders. The chapters have been revised to incorporate new text and figures. A companion CD containing case studies is also included.
Review
Practical Flow Cytometry by: Howard M. Shapiro publisher: Wiley-Liss, published: 2003-03-14 ISBN: 0471411256 sales rank: 597041
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From the reviews of the 3rd Edition? "The standard reference for anyone interested in understanding flow cytometry technology." -American Journal of Clinical Oncology "?one of the most valuable of its genre and?addressed to a wide audience?written in such an attractive way, being both informative and stimulating." -Trends in Cell Biology This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.
Review Skimmed it while loading samples as an undergrad researcher. From reading the intro, I guess previous editions actually showed you how to build your own flow cytometer. So for the uber-geek, older editions might be a great find. As for the newer edition, chock full of examples from peer review. Make sure you have a good background in instrumentation before picking it up.
Shapiro's book tries to be everything to everyone and if there ever was a book that succeeded doing so, this is it. Fully referenced (1000's of references) like an academic paper; containing all the information and explanations, from physics principles and implementations; to chemical structures and properties; to biological models and considerations; to practical uses and applications. It also contains an historical introduction that not only adds perspective but also enormous insight into the field. The book is fairly long, and as such maybe is not the best place for absolute beginners in the field. Nonetheless, knowing as much, the author introduced a chapter that is a briefer introduction to the field, allowing beginners to gain an initial solid footing before delving deeper. Anyone who uses flow cytometry on a regular basis, and certainly anyone using flow cytometry as an important research or clinical method should have this book and read it. You can always skip the parts that might not interest you or that are less relevant. In fact, originally I intended to do so, but once you begin to read you can't stop, it's almost like a novel. The author's witty, down to earth and extremely clear prose makes it easier to follow the denser scientific parts and make plain enjoyable and funny the other parts. He even adds poetry and music, showing you what it is to get joy from science. Importantly, as you read and learn about so many fields and areas related to flow cytometry and cytometry in general, you emerge with a much broader understanding of scientific principles and whole areas of scientific knowledge that are of prime importance to any scientist. It expands your horizons and explains the connections and interplay between different disciplines. Yes, it is that good. Shapiro's book is also full with tips, short protocols, ideas and implementations that have proven to be incredibly helpful to my scientific work. The reason my reviews title is '(Flow) cytometry...' is that after finishing the book you have really gained insight and knowledge on cytometry in general and not only on flow cytometry. Finally, even if not read cover to cover, this book is the definitive reference for all things (flow) cytometry, and as such has a place of honor in any biologist's library.
I am a medical doctor who oversees a flow cytometry clinical laboratory. I have always heard about Shapiro's flow cytometry textbook but had never reviewed it. After having done so, I can in all good consciousness say that this is one of the most poorly written books and one of the least organized. As a result, the few useful pieces of information in it are hard to find and certainly almost impossible to follow through because of his erratic style and thought flow process. My advice to anyone looking to get a basic flow cytometry book is to look elsewhere and do not make a mistake and buy this book.
Flow Cytometry by: John L. Carey et al publisher: American Society for Clinical Pathology, published: 2007-10-08 ISBN: 0891895485 sales rank: 466455
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The new edition of 'Flow Cytometry in Clinical Diagnosis' promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations. Drs. Carey and McCoy bring the new edition of 'Flow Cytometry in Clinical Diagnosis' alive with the bonus CD, which inlcudes data from actual flow analysis and patient samples for 39 cases. You'll receive a limited license to a site where you can analyze list mode data from real cases, evaluate the data and clinical information, and compare your analysis and interpretation with that of the case author.
Review
Flow Cytometry: First Principles by: Alice Longobardi Givan publisher: Wiley-Liss, published: 2001-07-23 ISBN: 0471382248 sales rank: 331580
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Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: * Assumes no prior knowledge of flow cytometry * Progresses with an informal, engaging lecture style from simpleto more complex concepts * Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis * Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Review Good for beginners/non-speicialists who want to know general principles in flow cytometry. It's easy to read and understand.
The book includes almost you need to know before starting flow cytometry. If anyone wants to broadly get the knowledge but not go into the detail of flow cytometry, it's a good choice. You probably can go through it in 1-2 days.
"I consider it to be the best presentation of principles of flow cytometry. The text is clearly written, the figures are well chosen." -- Zbigniew Darzynkiewicz, Brander Cancer Research Institute, New York Medical College
This text is a detailed guide to the use of flow cytometry, immunohistochemistry, and molecular genetic techniques for diagnosis of hematologic neoplasms. Dr. Sun explains the principles of these techniques and demonstrates their utility in 39 clinical cases covering all important entities. Each case represents a comprehensive diagnostic approach including a clinical history and flow cytometric, immunohistochemical, and molecular genetic findings. Abundant full-color illustrations show histologic sections, immunohistochemical stains, bone marrow, peripheral blood, and body fluid smears, and each case includes a complete set of flow cytometric histograms. Over 100 tables compare and differentiate the diagnostic features of similar diseases.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for:the preparation and handling of standards,reagents and specimenslaboratory analyses for immunophenotypingmeasurement of DNA content and cell cycle analysesdetermination of cell physiology and functionset up and operation of flow cytometerscomputational and data analysis techniquessafety precautionsDrawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user?s needs. Special features of Handbook of Flow Cytometry Methods include:ample space throughout for personal annotationssuggestions of alternative procedures; trouble-shooting tipsextensive list of reagents and supplierscomb-bound format for ease of use at the lab benchHandbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine.
Key features of this highly illustrated and practical guide include comprehensive coverage of the aspects relevant for clinical diagnosis, an explanation of the general parameters useful in diagnosis with flow cytometry, correlation with color histomorphological findings throughout, and a systematic approach from basic cases to complicated problem areas. All hematopathologists and neoplastic hematologists will find this an important resource for keeping up to date with developments in clinical practice.
Review The book contains essential knowledges and several pictures of dot plots correlated with histomorphology & immunohistochemistry, including tables showing immunophenotypic expression of any diseases. The chapters cover nearly all common diseases/problems in neoplastic hematology. This book is good for quick reference.
Co-Reading with "Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation" (by Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan), which contains practical knowledges and over 80 sample cases is recommended.
This book is a very handy tool every practicing Hematopathologist must have. The book has a nice 'flow'(no pun intended)and chapters are easy to follow.
Flow Cytometry for Biotechnology publisher: Oxford University Press, USA, published: 2005-09-02 ISBN: 0195152344 sales rank: 910594
Product Description
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.
American Red Cross, Rockville, MD. Offers reproducible flow cytometry methods using new technologies. Presents the principle behind the technique followed by step-by-step laboratory instructions. For researchers. Previous edition: c1999. Softcover, hardcover available and listed in 2004-18. DNLM: Flow Cytometry--methods.