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| By: Howard M. Shapiro ISBN: 0471411256 Publisher: Wiley-Liss Release Date: 14 March, 2003 Bioscience book rank: 90807 | Skimmed it while loading samples as an undergrad researcher. From reading the intro, I guess previous editions actually showed you how to build your own flow cytometer. So for the uber-geek, older editions might be a great find. As for the newer edition, chock full of examples from peer review. Make sure you have a good background in instrumentation before picking it up. Shapiro's book tries to be everything to everyone and if there ever was a book that succeeded doing so, this is it. Fully referenced (1000's of references) like an academic paper; containing all the information and explanations, from physics principles and implementations; to chemical structures and properties; to biological models and considerations; to practical uses and applications. It also contains an historical introduction that not only adds perspective but also enormous insight into the field. The book is fairly long, and as such maybe is not the best place for absolute beginners in the field. Nonetheless, knowing as much, the author introduced a chapter that is a briefer introduction to the field, allowing beginners to gain an initial solid footing before delving deeper. Anyone who uses flow cytometry on a regular basis, and certainly anyone using flow cytometry as an important research or clinical method should have this book and read it. You can always skip the parts that might not interest you or that are less relevant. In fact, originally I intended to do so, but once you begin to read you can't stop, it's almost like a novel. The author's witty, down to earth and extremely clear prose makes it easier to follow the denser scientific parts and make plain enjoyable and funny the other parts. He even adds poetry and music, showing you what it is to get joy from science. Importantly, as you read and learn about so many fields and areas related to flow cytometry and cytometry in general, you emerge with a much broader understanding of scientific principles and whole areas of scientific knowledge that are of prime importance to any scientist. It expands your horizons and explains the connections and interplay between different disciplines. Yes, it is that good. Shapiro's book is also full with tips, short protocols, ideas and implementations that have proven to be incredibly helpful to my scientific work. The reason my reviews title is '(Flow) cytometry...' is that after finishing the book you have really gained insight and knowledge on cytometry in general and not only on flow cytometry. Finally, even if not read cover to cover, this book is the definitive reference for all things (flow) cytometry, and as such has a place of honor in any biologist's library. I am a medical doctor who oversees a flow cytometry clinical laboratory. I have always heard about Shapiro's flow cytometry textbook but had never reviewed it. After having done so, I can in all good consciousness say that this is one of the most poorly written books and one of the least organized. As a result, the few useful pieces of information in it are hard to find and certainly almost impossible to follow through because of his erratic style and thought flow process. My advice to anyone looking to get a basic flow cytometry book is to look elsewhere and do not make a mistake and buy this book. | |
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| By: Wojciech Gorczyca ISBN: 1841845655 Publisher: Informa Healthcare Release Date: 03 March, 2006 Bioscience book rank: 195895 | The book contains essential knowledges and several pictures of dot plots correlated with histomorphology & immunohistochemistry, including tables showing immunophenotypic expression of any diseases. The chapters cover nearly all common diseases/problems in neoplastic hematology. This book is good for quick reference.
<br />Co-Reading with "Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation" (by Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan), which contains practical knowledges and over 80 sample cases is recommended. This book is a very handy tool every practicing Hematopathologist must have. The book has a nice 'flow'(no pun intended)and chapters are easy to follow. | |
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| By: James V. Watson ISBN: 0521611997 Publisher: Cambridge University Press Release Date: 11 November, 2004 Bioscience book rank: 219298 | ||
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| By: Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan ISBN: 1588298558 Publisher: Humana Press Release Date: 05 September, 2007 Bioscience book rank: 111539 | ||
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| By: Alice Longobardi Givan ISBN: 0471382248 Publisher: Wiley-Liss Release Date: 23 July, 2001 Bioscience book rank: 58303 | "I consider it to be the best presentation of principles of flow cytometry. The text is clearly written, the figures are well chosen." -- Zbigniew Darzynkiewicz, Brander Cancer Research Institute, New York Medical College A core book designed mainly for the nonspecialist, "Flow Cytometry: First Principles" is addressing in ten chapters the essential issues in flow-cytometry, from flow machines to reagents, techniques and their applications in fundamental research and clinical settings. The first chapters conveys us as back as the beginning of the century, when the concept of flow-cytometry germinated. Light microscopy, cell counters and ink jet technology are standing at the grounds of flow-cytometry and their merging paved the way toward the modern, "black box", flow cytometers. However, "beyond the black box", the instrumentation and the technical aspects are comprehensively described in several chapters. a discussion on data acquisition and analysis is provided, along with the most used cell staining procedures, fluorochromes, lenses, mirrors and lasers. A widespread application in flow-cytometry is cell labelling, particularly leukocyte subpopulations identification with monoclonal antibodies binding cell surface specific markers. Although lymphocytes are the prototype, methods presented here -dot-plot, histogram, quadrant analysis and gating are applicable to many other cell types , including bacteria or algae. With the advent of some "state of the art" machines, DNA and chromosome analyses become feasible by flow-cytometry. It is foreseeable that molecular biology on flow-cytometer will not stop to cell cycle, ploidy and apoptosis investigation, but even chromosomal translocations or point mutations would be detectable with enough precision for diagnosis. Beside fundamental research applications, flow-cytometry is now an indispensable technique in the clinical laboratory, too. Oncology, immunology, hematology and pathology are fields where flow diagnosis has sometimes the last word to say. Cross-match analysis and HLA phenotyping can now rapidly be performed by flow-cytometry, with usefulness in organ and bone-marrow transplantation. The final chapter is intended to present the flow-cytometry of the future, as seen in 1992, with an emphasis on the functional assays which are gaining now an increasing interest. However, a precedent, "out of the stream" chapter is preparing us on what this future could look alike. It approaches some of the border-line pplications, in fields where flow-cytometry is becoming a current tool of research. These are microbiology -prokaryotic DNA and drug resistance determination-, ecology -aquatic environment, plankton, algae-, developmental biology or the functional studies -Ca2+influx. The book is highly recommended for flow-cytometry and sorting beginners, but it is an equally pleasant lecture for people working in many life sciences fields, eager to find out how flow-cytometry is shaping current biological research. | |
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| By: Tsieh Sun ISBN: 078178400X Publisher: Lippincott Williams & Wilkins Release Date: 01 October, 2007 Bioscience book rank: 592995 | ||
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| By: James V. Watson ISBN: 0521019702 Publisher: Cambridge University Press Release Date: 15 September, 2005 Bioscience book rank: 414887 | ||
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| By: Marion G. Macey ISBN: 1588296911 Publisher: Humana Press Release Date: 03 May, 2007 Bioscience book rank: 186152 | ||
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| By: Teresa S. Hawley, Robert G. Hawley ISBN: 1588292355 Publisher: Humana Press Release Date: 18 March, 2004 Bioscience book rank: 949962 | ||
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| By: Larry A. Sklar ISBN: 0195152344 Publisher: Oxford University Press, USA Release Date: 02 September, 2005 Bioscience book rank: 873345 | ||
Related books in this category:
cell culture, methods in cell biology, apoptosis, cell cycle, mitosis, signal transduction, receptor, mitochondria, ribosome, stem cell, flow cytometry
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