epitope-mapping books

Related books on 'epitope-mapping'

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Epitope Mapping Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 1996-08-01
ASIN: B001DA9OLC
sales rank: 995615
Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping-all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. A method for T-cell epitopes, instructions on using "natural" variants or mutants, and techniques for producing panels of antibodies for epitope mapping are also included.

Epitope Mapping Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-05-20
ASIN: 1934115177
EAN: 9781934115176
sales rank: 2222189
Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

Epitope Mapping: A Practical Approach

publisher: Oxford University Press, USA, published: 2001-03-15
ASIN: 0199636524
EAN: 9780199636525
sales rank: 2496108
Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotoxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific epitope. Investigation of carbohydrates can also be useful to epitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.

Peptide Arrays on Membrane Supports: Synthesis and Applications (Springer Lab Manuals)

publisher: Springer, published: 2010-12-15
ASIN: 3642076394
EAN: 9783642076398
sales rank: 4577674
Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.

Gene Isolation and Mapping Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 1996-10-19
ASIN: 0896034828
EAN: 9780896034822
sales rank: 4439563
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Genome Mapping and Genomics in Animal-Associated Microbes

publisher: Springer, published: 2010-11-10
ASIN: 3642093361
EAN: 9783642093364
Achievements and progress in genome mapping and the genomics of microbes supersede by far those for higher plants and animals, in part due to their enormous economic implication but also smaller genome size. In the post-genomic era, whole genome sequences of animal-associated microbes are providing clues to depicting the genetic basis of the complex host-pathogen relationships and the evolution of parasitism; and to improving methods of controlling pathogens. This volume focuses on a globally important group of intracellular prokaryotic pathogens which affect livestock animals. These include Brucella, Mycobacterium, Anaplasma and Ehrlichia, as well as the protozoan pathogens Cryptosporidium and Theileria, for which genome sequence data is available. Insights from comparative genomics of the microbes described provide clues to the adaptation involved in host-microbe interactions, as well as resources potentially useful for application in future research and product development.

Epitope Mapping Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-01-20
ASIN: 1617379247
EAN: 9781617379246
sales rank: 5614650
Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

Synthetic peptides: Epitope Mapping and Applications: Synthetic peptide based epitope mapping and development of safe diagnostics for Infectious Bursal Disease Virus(IBDV)

by: Saravanan Paramasivam
publisher: LAP LAMBERT Academic Publishing, published: 2012-01-10
ASIN: 384733297X
EAN: 9783847332978
This book describes basics of synthetic peptide technology and its usage in epitope mapping for disease causing viruses of human, animals and birds.It is handy for newcomers on how to analyse the genomic data of viruses and predict antigenic determinants also called as epitopes in structural proteins of viruses and how to fish out promising epitopes.The methods for using computer alogorithms like DNASTAR software and synthesizing peptides by simple methods were described lucidly.Preparation of multiple antigenic peptides,screening of peptides representing epitopes were stated very elaborately.Most importantly,the methods for producing antipeptide antibody in rabbits without using any carrier proteins which in turn will produce high titred sera was written in this book in simple language.Purification of sera by sepharose methods was also included in this book.This book is a compendium of all methods for Molecular Biology and Molecular Virology students,researchers and scientists.It is expected to fill the gap in synthetic peptide technology and its application for disease diagnosis and developing novel,safe,non-infectious immunogens as vaccines for controlling animal viral diseases.

Guide to Human Genome Computing, Second Edition

publisher: Academic Press, published: 1998-04-21
ASIN: 0121020517
EAN: 9780121020514
sales rank: 155447
The Guide to Human Genome Computing is invaluable to scientists who wish to make use of the powerful computing tools now available to assist them in the field of human genome analysis. This book clearly explains access and use of sequence databases, and presents the various computer packages used to analyze DNA sequences, measure linkage analysis, compare and align DNA sequences from different genes or organisms, and infer structural and functional information about proteins from sequence data.
This Second Edition contains completely updated material. Rather than a revision of the previous volume, the Second Edition is essentially a new book, based on the subjects which will be of interest over the coming years. This new book is international, both in scope and authorship.

Computing resources for the following are clearly explained:
* Internet resources - databases etc.
* Genetic analysis
* Sib-pair studies
* Comparative mapping
* Radiation hybrids
* Sequence ready clone maps
* Human genome sequencing
* ESTs
* Gene prediction
* Gene expression

Comparative Gene Finding: Models, Algorithms and Implementation (Computational Biology)

by: Marina Axelson-Fisk
publisher: Springer, published: 2010-02-17
ASIN: 1849961034
EAN: 9781849961035
sales rank: 3079738
This unique text/reference describes the state of the art in computational gene finding, with a particular focus on comparative approaches. Providing both an overview of the various methods that are applied in the field, and a concise guide on how computational gene finders are built, the book covers a broad range of topics from probability theory, statistics, information theory, optimization theory and numerical analysis. Features: describes how algorithms and sequence alignments can be combined to improve the accuracy of gene finding; introduces the basic biological terms and concepts in genetics, and provides an historical overview of algorithm development; explores the gene features most commonly captured by a computational gene model; discusses the algorithms most commonly used for single-species gene finding; investigates approaches to pairwise and multiple sequence alignments; explains the basics of parameter training; illustrates how to implement a comparative gene finder.

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	Epitope Mapping Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 1996-08-01 ASIN: B001DA9OLC sales rank: 995615 Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping-all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. A method for T-cell epitopes, instructions on using "natural" variants or mutants, and techniques for producing panels of antibodies for epitope mapping are also included.
	Epitope Mapping Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-05-20 ASIN: 1934115177 EAN: 9781934115176 sales rank: 2222189 Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
	Epitope Mapping: A Practical Approach publisher: Oxford University Press, USA, published: 2001-03-15 ASIN: 0199636524 EAN: 9780199636525 sales rank: 2496108 Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotoxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific epitope. Investigation of carbohydrates can also be useful to epitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.
	Peptide Arrays on Membrane Supports: Synthesis and Applications (Springer Lab Manuals) publisher: Springer, published: 2010-12-15 ASIN: 3642076394 EAN: 9783642076398 sales rank: 4577674 Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.
	Gene Isolation and Mapping Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 1996-10-19 ASIN: 0896034828 EAN: 9780896034822 sales rank: 4439563 An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.
	Genome Mapping and Genomics in Animal-Associated Microbes publisher: Springer, published: 2010-11-10 ASIN: 3642093361 EAN: 9783642093364 Achievements and progress in genome mapping and the genomics of microbes supersede by far those for higher plants and animals, in part due to their enormous economic implication but also smaller genome size. In the post-genomic era, whole genome sequences of animal-associated microbes are providing clues to depicting the genetic basis of the complex host-pathogen relationships and the evolution of parasitism; and to improving methods of controlling pathogens. This volume focuses on a globally important group of intracellular prokaryotic pathogens which affect livestock animals. These include Brucella, Mycobacterium, Anaplasma and Ehrlichia, as well as the protozoan pathogens Cryptosporidium and Theileria, for which genome sequence data is available. Insights from comparative genomics of the microbes described provide clues to the adaptation involved in host-microbe interactions, as well as resources potentially useful for application in future research and product development.
	Epitope Mapping Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-01-20 ASIN: 1617379247 EAN: 9781617379246 sales rank: 5614650 Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
	Synthetic peptides: Epitope Mapping and Applications: Synthetic peptide based epitope mapping and development of safe diagnostics for Infectious Bursal Disease Virus(IBDV) by: Saravanan Paramasivam publisher: LAP LAMBERT Academic Publishing, published: 2012-01-10 ASIN: 384733297X EAN: 9783847332978 This book describes basics of synthetic peptide technology and its usage in epitope mapping for disease causing viruses of human, animals and birds.It is handy for newcomers on how to analyse the genomic data of viruses and predict antigenic determinants also called as epitopes in structural proteins of viruses and how to fish out promising epitopes.The methods for using computer alogorithms like DNASTAR software and synthesizing peptides by simple methods were described lucidly.Preparation of multiple antigenic peptides,screening of peptides representing epitopes were stated very elaborately.Most importantly,the methods for producing antipeptide antibody in rabbits without using any carrier proteins which in turn will produce high titred sera was written in this book in simple language.Purification of sera by sepharose methods was also included in this book.This book is a compendium of all methods for Molecular Biology and Molecular Virology students,researchers and scientists.It is expected to fill the gap in synthetic peptide technology and its application for disease diagnosis and developing novel,safe,non-infectious immunogens as vaccines for controlling animal viral diseases.
	Guide to Human Genome Computing, Second Edition publisher: Academic Press, published: 1998-04-21 ASIN: 0121020517 EAN: 9780121020514 sales rank: 155447 The Guide to Human Genome Computing is invaluable to scientists who wish to make use of the powerful computing tools now available to assist them in the field of human genome analysis. This book clearly explains access and use of sequence databases, and presents the various computer packages used to analyze DNA sequences, measure linkage analysis, compare and align DNA sequences from different genes or organisms, and infer structural and functional information about proteins from sequence data. This Second Edition contains completely updated material. Rather than a revision of the previous volume, the Second Edition is essentially a new book, based on the subjects which will be of interest over the coming years. This new book is international, both in scope and authorship. Computing resources for the following are clearly explained: * Internet resources - databases etc. * Genetic analysis * Sib-pair studies * Comparative mapping * Radiation hybrids * Sequence ready clone maps * Human genome sequencing * ESTs * Gene prediction * Gene expression
	Comparative Gene Finding: Models, Algorithms and Implementation (Computational Biology) by: Marina Axelson-Fisk publisher: Springer, published: 2010-02-17 ASIN: 1849961034 EAN: 9781849961035 sales rank: 3079738 This unique text/reference describes the state of the art in computational gene finding, with a particular focus on comparative approaches. Providing both an overview of the various methods that are applied in the field, and a concise guide on how computational gene finders are built, the book covers a broad range of topics from probability theory, statistics, information theory, optimization theory and numerical analysis. Features: describes how algorithms and sequence alignments can be combined to improve the accuracy of gene finding; introduces the basic biological terms and concepts in genetics, and provides an historical overview of algorithm development; explores the gene features most commonly captured by a computational gene model; discusses the algorithms most commonly used for single-species gene finding; investigates approaches to pairwise and multiple sequence alignments; explains the basics of parameter training; illustrates how to implement a comparative gene finder.