Books on 'differential-display'
Total books: 10 Page 1 of 1
publisher: Oxford University Press, USA, published: 2000-05-15
ISBN: 019963758X
sales rank: 5124232
Product Description
Smith Kline Beecham Pharmaceuticals, Harlow, UK. Details the application of RT-PCR differential display to a range of experimental biological systems, including single cells. Coverage includes recent advances in fluorescent differential display, experimental design for analysis of complex tissues, and more. Softcover, hardcover available.
Review
publisher: Eaton Publishing Company/Biotechniques Books, published: 1999-03
ISBN: 1881299317
sales rank: 7755726
Product Description
This volume includes 59 key papers on differential display and related technologies selected from the pages of BioTechniques journal by two pioneers of this important expression methodology -- Dr. Arthur Pardee, the creator of differential display, and Dr. Michael McClelland who developed the closely related AP-PCR technique. A special feature of this book is the original review chapter prepared by Drs. Pardee and McClelland, commenting on the state of differential display today. To provide the most current information possible, many articles include "Updates" prepared by their original authors. This volume encompasses many important modifications made to the differential display in recent years and will provide the reader with a good overview of the technique and a broad array of useful protocols.
Review
publisher: Humana Press, published: 2005-10-21
ISBN: 1588293386
sales rank: 2393331
Product Description
Based on a new mathematical model of differential display (DD), this updated second edition takes advantage of automation, as well as digital data acquisition and analysis. The authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display (FD) image, and restriction fragment-based DD screenings. Other improvements discussed are the combining of DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction.
Review
by: Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
publisher: Springer, published: 1998-03-05
ISBN: 3540632972
sales rank: 6194387
Product Description
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
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by: Ron A. (Edt)/ Robertson, Harry A Leslie
publisher: Oxford University Press, USA, published: 2000
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by: Peng Liang
publisher: NY, published: 1980
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publisher: Alphascript Publishing, published: 2009-12-21
ISBN: 6130218176
sales rank: 514007
Product Description
High Quality Content by WIKIPEDIA articles! High Quality Content by WIKIPEDIA articles! High Quality Content by WIKIPEDIA articles! HDMI (High-Definition Multimedia Interface) is a compact audio/video interface for transmitting uncompressed digital data. It represents a digital alternative to consumer analog standards, such as radio frequency (RF) coaxial cable, composite video, S-Video, SCART, component video, D-Terminal, and VGA. HDMI connects digital audio/video sources?such as set-top boxes, Blu-ray Disc players, personal computers (PCs), video game consoles (such as the PlayStation 3 and some models of Xbox 360), and AV receivers?to compatible digital audio devices, computer monitors, and digital televisions. HDMI supports, on a single cable, any TV or PC video format, including standard, enhanced, and high-definition video; up to 8 channels of digital audio; and a Consumer Electronics Control (CEC) connection. The CEC allows HDMI devices to control each other when necessary and allows the user to operate multiple devices with one remote control handset. Because HDMI is electrically compatible with the signals used by Digital Visual Interface (DVI), no signal conversion is necessary, nor is there a loss of video quality when a DVI-to-HDMI adapter is used.
Review
by: Susanne Wasner, Christian Sailer
publisher: Borm Bruckmeier Publishing LLC, published: 2002-10
ISBN: 1591033012
Product Description
Value package contains pocket-sized quick reference guides to more than 800 lists of symptoms, physical signs, and abnormal findings. Keywords are listed in alphabetical order for easy access. Covers all medical specialties. For students, residents, nurses, and other healthcare professionals. Individual text also available. Softcover.
Review
by: L. Potenza, L. Ubaldi, R. De Sanctis, R De Bellis
publisher: Elsevier, published: 2004-07-11
Product Description
This digital document is a journal article from Mut.Res.-Genetic Toxicology and Environmental Mutagenesis, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: Escherichia coli cultures exposed to a 300mT static magnetic field (SMF) were studied in order to analyse possible induced changes in cellular growth and gene expression. Biomass was evaluated by visible-light spectrometry and gene expression analyses were carried out by use of RNA arbitrarily primed PCR. The bacterial strain XL-1Blue, cultivated in traditional and modified Luria-Bertani medium, was exposed to SMF generated by permanent neodymium magnetic disks. The results show alterations induced by SMF in terms of increased cell proliferation and changes in gene expression compared with control groups. Three cDNAs were found to be expressed only in the exposed cells, whereas one cDNA was more expressed in the controls. One clone, expressed only in the exposed cells, corresponds to a putative transposase. This is of particular interest in that it suggests that exposure to a magnetic field may stimulate transposition activity.
Review
by: Christopher J. Bielik
publisher: Storming Media, published: 1999
ISBN: 1423543742
Product Description
This is a NAVAL POSTGRADUATE SCHOOL MONTEREY CA report procured by the Pentagon and made available for public release. It has been reproduced in the best form available to the Pentagon. It is not spiral-bound, but rather assembled with Velobinding in a soft, white linen cover. The Storming Media report number is A724663. The abstract provided by the Pentagon follows: This thesis examines the potential impact of eliminating out-of- pocket housing expenses on the quality of life and retention decisions of Navy families living in public/private venture housing. The Differential lease Payment (DLP) program, an experimental Department of Defense pilot project, is on the verge of implementation at Naval Station Everett, WA. The main research question and hypothesis is that removing out-of-pocket housing expenses will substantially improve quality of life and positively affect reenlistment decisions of the impacted military families. A written questionnaire was administered to 71 military members and spouses living at the Country Manor public/private venture housing complex, and 25 semi-structured interviews were conducted from the 71 person sample. The study revealed at the .01 level of confidence that eliminating out-of-pocket housing expenses will substantially improve quality of life and will likely have a positive effect on reenlistment decisions. A related finding is that eliminating out-of-pocket housing expenses, although important, is less important than improving retirement benefits in terms of motivating reenlistment decisions. Recommendations are to implement the DLP program as soon as possible; improve retirement benefits to maximize retention; and ensure measurement of customer (military family) satisfaction is inte al to all future public/private ventures.
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methods in molecular biology, PCR, RT-PCR and real-time quantitative PCR, Differential Display, recombinant DNA, gene therapy, virus protocols, lentivirus methods, gene targeting, mouse knock-out and knock-in, transgenic technology, phenotyping, gene delivery and transfer, transcriptional regulation, RNA methods, RNA Polymerase, gene expression, protein translation regulation, protein kinase, protein phosphorylation, genomics, genomics methods, epigenetics, DNA methylation, DNA sequencing, RNA interference, microarray
Total 10 books of 1 pages