Related books on 'cell-culture tissue-culture'
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by: R. Ian Freshney publisher: Wiley-Blackwell, published: 2010-09-17 ASIN: 0470528125 EAN: 9780470528129 sales rank: 48910 This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams. What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition? I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book. What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure. What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated. What advice would you offer to scientists writing or editing their first book? - Determine the precise niche that you wish to fill. - Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands. - Ensure the text is well illustrated with tables and diagrams. - Make sure the book is properly cross-referenced. - Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not. How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions. Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago? Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation. How do you envision the evolution of science writing over the next 5-10 years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online. What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo. What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts. |
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by: Michael A. Dirr publisher: Timber Press, published: 2006-01-07 ASIN: 1604690046 EAN: 9781604690040 sales rank: 47376 Compiled by two distinguished professors of horticulture, The Reference Manual of Woody Plant Propagation is a must for professionals and students of horticulture. Over 1,100 species and their propagation requirements by seeds, cuttings, grafting and budding, and tissue culture are discussed in exhaustive detail. Essentially a recipe book for making more trees and shrubs, this reference is a high-level how-to. |
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publisher: Wiley, published: 2011-05-10 ASIN: 0470666587 EAN: 9780470666586 sales rank: 794532 This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable. |
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by: Bernice M. Martin publisher: Birkhäuser Boston, published: 1994-08-01 ASIN: 0817636439 EAN: 9780817636432 sales rank: 609307 Designed to teach the fundamentals of tissue culture, this text covers all aspects of the field, taking safety and sterility as key underlying themes. Containing trouble-shooting tips and problem sets, the book should give the novice a firm grasp of tissue culture and manipulations. |
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by: A., Griffiths, J.B. Doyle publisher: Wiley, published: 1998-12-16 ASIN: 0471982555 EAN: 9780471982555 sales rank: 1269668 Cell and Tissue Culture: Laboratory Procedures in Biotechnology Edited by Alan Doyle Centre for Applied Microbiology & Research, Porton Down, Salisbury, UK. and J. Bryan Griffiths Scientific Consultancy & Publishing, Porton, Salisbury, UK. Cell and Tissue Culture: Laboratory Procedures in Biotechnology introduces the reader to animal cell culture methods describing the key cells, core techniques, how to scale up the culture for commercial production, and regulatory aspects. This book provides easy to follow, step-by-step protocols, with trouble-shooting tips and notes on time considerations. Alternative procedures, background information and references supplement the main procedures described. Other features include: * Experimental examples to indicate expected results; * Quick reference symbols such as safety icons with warning notes; and, * A list of suppliers is provided to allow easy access to laboratory products. Written by a team of international scientists, Cell and Tissue Culture: Laboratory Procedures in Biotechnology will be of interest to researchers, technicians and process engineers using cell culture within the biotechnology, biomedicine and pharmaceutical industries. |
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by: Lydiane Kyte publisher: Timber Press, published: 1996-10-28 ASIN: 0881923613 EAN: 9780881923612 sales rank: 184905 Acclaimed since its first appearance as the most practical guide to plant tissue culture and widely adopted as a textbook, this standard work is now even better. This expanded edition introduces new developments in biotechnology, such as genetic engineering and cell culture. It provides detailed recipes for propagating plants from more than 30 families. It explains clearly how to set up a propagating laboratory, from a hobbyist's kitchen to an elaborate commercial enterprise. |
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by: Michael Butler publisher: Taylor & Francis, published: 2004-03-10 ASIN: 1859960499 EAN: 9781859960493 sales rank: 196032 Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use. |
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by: Michael R. Davey publisher: Wiley, published: 2010-05-18 ASIN: 0470686480 EAN: 9780470686485 sales rank: 1438703 The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.
An essential laboratory manual for students and early-career researchers. |
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by: Lewis Thomas publisher: Penguin (Non-Classics), published: 1978-02-23 ASIN: 0140047433 EAN: 9780140047431 sales rank: 35780 Elegant, suggestive, and clarifying, Lewis Thomas's profoundly humane vision explores the world around us and examines the complex interdependence of all things. Extending beyond the usual limitations of biological science and into a vast and wondrous world of hidden relationships, this provocative book explores in personal, poetic essays to topics such as computers, germs, language, music, death, insects, and medicine. Lewis Thomas writes, "Once you have become permanently startled, as I am, by the realization that we are a social species, you tend to keep an eye out for the pieces of evidence that this is, by and large, good for us." |
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publisher: Humana Press, published: 2011-11-05 ASIN: 1617793663 EAN: 9781617793660 sales rank: 850744 Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research. |
