antibody-protocol books

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Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology, Vol. 378)

publisher: Humana Press, published: 2007-06-25
ASIN: 1588295672
EAN: 9781588295675
sales rank: 528994
This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Therapeutic Antibodies: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-03-05
ASIN: 1934115924
EAN: 9781934115923
sales rank: 1194818
With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.

Antibodies: A Laboratory Manual

by: Ed Harlow
publisher: Cold Spring Harbor Laboratory Press, published: 1988-12-01
ASIN: 0879693142
EAN: 9780879693145
sales rank: 214395
"[Antibodies aims] to put current immunological methodology into the hands of non-specialists. Happily, Harlow and Lane have succeeded admirably and this manual seems certain also to gain a place on the laboratory bench (too useful to remain on the bookshelf) of a wide variety of biologistseven immunologists. The volume opens with four introductory chapters summarizing key features of the immune response; these provide a succinct overview of the field and are very well written. The scope of the rest of the book includes techniques of immunization and bleeding, monoclonal antibodies and hybridomas, protein labelling, immunoassays (mostly those using solid supports), immunoprecipitation of cellular antigens, immunoblotting and immunoaffinity purification. The methods are presented as easy-to-follow '1-2-3 protocols,' and assume relatively little experience. Rationales for procedures are often presented, which helps to remove some of the mystique that has been associated with immunological methodology."

Antibody Engineering: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617373524
EAN: 9781617373527
sales rank: 2951277
A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Alternative pre-mRNA Splicing

publisher: Wiley-VCH, published: 2012-02-14
ASIN: 3527326065
EAN: 9783527326068
sales rank: 1112019
All eukaryotic messenger RNAs are derived from precursors, the pre-mRNAs, by an extensive series of nuclear modifi cations. Most notably, the removal of introns and the joining of the remaining exons in the process of pre-mRNA splicing is essential to generate mRNAs that are suitable templates for translation. The vast majority of human pre-mRNAs undergo alternative splicing, in which different RNA parts are either included or omitted in the mature mRNA. This process allows individual genes to generate multiple proteins and makes alternative pre-mRNA splicing a central element in gene regulation. Although pre-mRNA splicing occurs with high fi delity, an increasing number of diseases have been linked to defects that lead to mis-spliced mRNAs.
This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing.
A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing.

Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617376469
EAN: 9781617376467
sales rank: 2787041
This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Recombinant Antibodies for Cancer Therapy: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2011-10-08
ASIN: 1617372501
EAN: 9781617372506
sales rank: 4460870
In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschoff and Jürgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of novel anticancer therapeutics based on recombinant antibody technology. These readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.

Monoclonal Antibody Protocols (Methods in Molecular Biology, Vol 45)

publisher: Humana Press, published: 1995-05-23
ASIN: 0896033082
EAN: 9780896033085
sales rank: 4780574
Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.

RNA-Protein Interaction Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617375551
EAN: 9781617375552
sales rank: 3695969
Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.

In Situ Hybridization Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-12-09
ASIN: 1617375446
EAN: 9781617375446
sales rank: 2561089
This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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	Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology, Vol. 378) publisher: Humana Press, published: 2007-06-25 ASIN: 1588295672 EAN: 9781588295675 sales rank: 528994 This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.
	Therapeutic Antibodies: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-03-05 ASIN: 1934115924 EAN: 9781934115923 sales rank: 1194818 With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.
	Antibodies: A Laboratory Manual by: Ed Harlow publisher: Cold Spring Harbor Laboratory Press, published: 1988-12-01 ASIN: 0879693142 EAN: 9780879693145 sales rank: 214395 "[Antibodies aims] to put current immunological methodology into the hands of non-specialists. Happily, Harlow and Lane have succeeded admirably and this manual seems certain also to gain a place on the laboratory bench (too useful to remain on the bookshelf) of a wide variety of biologistseven immunologists. The volume opens with four introductory chapters summarizing key features of the immune response; these provide a succinct overview of the field and are very well written. The scope of the rest of the book includes techniques of immunization and bleeding, monoclonal antibodies and hybridomas, protein labelling, immunoassays (mostly those using solid supports), immunoprecipitation of cellular antigens, immunoblotting and immunoaffinity purification. The methods are presented as easy-to-follow '1-2-3 protocols,' and assume relatively little experience. Rationales for procedures are often presented, which helps to remove some of the mystique that has been associated with immunological methodology."
	Antibody Engineering: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617373524 EAN: 9781617373527 sales rank: 2951277 A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.
	Alternative pre-mRNA Splicing publisher: Wiley-VCH, published: 2012-02-14 ASIN: 3527326065 EAN: 9783527326068 sales rank: 1112019 All eukaryotic messenger RNAs are derived from precursors, the pre-mRNAs, by an extensive series of nuclear modifi cations. Most notably, the removal of introns and the joining of the remaining exons in the process of pre-mRNA splicing is essential to generate mRNAs that are suitable templates for translation. The vast majority of human pre-mRNAs undergo alternative splicing, in which different RNA parts are either included or omitted in the mature mRNA. This process allows individual genes to generate multiple proteins and makes alternative pre-mRNA splicing a central element in gene regulation. Although pre-mRNA splicing occurs with high fi delity, an increasing number of diseases have been linked to defects that lead to mis-spliced mRNAs. This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing.
	Monoclonal Antibodies: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617376469 EAN: 9781617376467 sales rank: 2787041 This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.
	Recombinant Antibodies for Cancer Therapy: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2011-10-08 ASIN: 1617372501 EAN: 9781617372506 sales rank: 4460870 In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschoff and Jürgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of novel anticancer therapeutics based on recombinant antibody technology. These readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.
	Monoclonal Antibody Protocols (Methods in Molecular Biology, Vol 45) publisher: Humana Press, published: 1995-05-23 ASIN: 0896033082 EAN: 9780896033085 sales rank: 4780574 Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.
	RNA-Protein Interaction Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617375551 EAN: 9781617375552 sales rank: 3695969 Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.
	In Situ Hybridization Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-12-09 ASIN: 1617375446 EAN: 9781617375446 sales rank: 2561089 This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.