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by: Alan S. Gerstein
publisher: Wiley-Liss, published: 2001-08-24
ASIN: 0471379727
EAN: 9780471379720
sales rank: 1299207
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as: - Have you selected the most appropriate research strategy?
- Have you identified the issues critical to your successful application of a technique?
- Are you familiar with the limitations of a given technique?
- When should common procedural rules of thumb not be applied?
- What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include: - Working safely with biological samples and radioactive materials
- DNA and RNA purification
- PCR
- Protein and nucleid acid hybridization
- Prokaryotic and eukaryotic expression systems
- Properly using and maintaining laboratory equipment
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publisher: Informa Healthcare, published: 2005-03-29
ASIN: 0824726677
EAN: 9780824726676
sales rank: 1344874
This reference assists scientists in the selection and application of analytical techniques for biopharmaceutical identification, formulation, and assessment-providing a valuable survey of exploratory methods commonly utilized during the preclinical, clinical, and commercial phases of development.
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by: Gerstein
publisher: Co-CBS, published: 2001-01-01
ASIN: B0049VGUCI
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publisher: International University Line, published: 2009-04-15
ASIN: 0972077448
EAN: 9780972077446
sales rank: 693747
Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the Practical Protein Crystallization courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere. The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench.
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by: Richard Kowles
publisher: Springer, published: 2001-06-21
ASIN: 0387988408
EAN: 9780387988405
sales rank: 9058166
Helping undergraduates in the analysis of genetic problems, this work emphasizes solutions, not just answers. The strategy is to provide the student with the essential steps and the reasoning involved in conducting the analysis, and throughout the book, an attempt is made to present a balanced account of genetics. Topics, therefore, center about Mendelian, cytogenetic, molecular, quantitative, and population genetics, with a few more specialized areas. Whenever possible, the student is provided with the appropriate basic statistics necessary to make some the analyses. The book also builds on itself; that is, analytical methods learned in early parts of the book are subsequently revisited and used for later analyses. A deliberate attempt is made to make complex concepts simple, and sometimes to point out that apparently simple concepts are sometimes less so on further investigation. Any student taking a genetics course will find this an invaluable aid to achieving a good understanding of genetic principles and practice.
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by: Antonios Matsakas
publisher: Diplomarbeiten Agentur diplom.de, published: 2004-01-01
ASIN: 3838682939
EAN: 9783838682938
Dissertation / Doktorarbeit, die am 12.07.2004 erfolgreich an einer Sporthochschule in Deutschland im Fachbereich Medizin- und Naturwissenschaften eingereicht wurde. Abstract: Skeletal muscle is a complex and heterogeneous tissue capable of remarkable ad
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by: Isumo Bergmann
publisher: Gabler Verlag, published: 2011-05-12
ASIN: 3834928984
EAN: 9783834928986
Isumo Bergmann entwickelt semantische Patentanalysen, die die Ähnlichkeiten zwischen Patenten aufdecken sollen. Er gestaltet den Patentrecherche- und -analyseprozess für biotechnologische Fragestellungen neu und wertet ausgewählte Patentverletzungsfälle in der Biotechnologie aus.
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