Basic Biostatistics presents a multidisciplinary survey of biostatics methods, each illustrated with hands-on examples. Methods range from the elementary, including descriptive statistics, study design, statistical interference, categorical variables, evaluation of diagnostic tests, comparison of means, linear regression, and logistic regression. These introductory methods create a portfolio of biostatistical techniques for both novice and expert researchers. More complicated statistical methods are introduced as well, including those requiring either collaboration with a biostatistician or the use of a statistical package. Specific topics of interest include microarray analysis, missing data techniques, power and sample size, statistical methods in genetics. Expert advice is given on when to seek statistical help, and how to conduct a meeting with the statistical collaborator or consultant. Basic Biostatistics is an essential resource for researchers at every level of their career.
Plant Secondary Metabolites provides reliable assays that will equip researchers to meet the unprecedented challenge of fulfilling the huge demand for feed driven by increasing demand of animal protein in developing countries. Using more than a decade of experience working with the quantification of plant secondary metabolites, the authors have created a manual allowing for the safe and efficient use of locally available feed resources. This comprehensive new volume also offers readers a better understanding of plant-animal interactions. The methodologies provided in this volume may also be used to determine plant secondary metabolites in human food and in studying the implications of their consumption on human health and welfare. This volume contains methods for analysis of some important plant secondary metabolites, which may be conducted in laboratories equipped with basic facilities. The chemical nature of plant secondary metabolites and their known physiological effects and mechanisms of action are also briefly presented. The methods have been written in a recipe-like format designed for direct practical use in the laboratory. Authoritative and extensive, Plant Secondary Metabolites will become an essential reference for plant biologists, biochemists, and molecular and cell biologists.
Population studies and epidemiology facilitate the discovery of genetic and environmental determinants of cancer and the development of new approaches to cancer control and prevention, therefore they play a central role in the creation of health policies. Cancer Epidemiology compiles areas of research which cover etiological factors or determinants that contribute to the development of cancer and describe the latest technologies in cancer epidemiology. In Volume 1, Host Susceptibility Factors, leading experts provide chapters on cancer incidence, prevalence, mortality and surveillance, methods, technologies and study design in cancer epidemiology as well as host susceptibility factors in cancer epidemiology. Although a non-standard volume of the highly successful Methods in Molecular Biology? series, this comprehensive text retains the commitment of the series to collecting the kind of detailed, up-to-date information and implementation advice that is crucial for getting optimal results. Cutting-edge and essential, Cancer Epidemiology allows readers to get the maximum advantage of the methods involved in this exciting and important field.
University of Connecticut, Farmington. Methods in Molecular Biology, Volume 15. Selective manual of analytical and preparative protocols in polymer chain reaction (PCR). Plastic spiral binding. 67 contributors, mostly U.S.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. A companion CD provides electronic color versions of all illustrations in the book. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
With molecular biology now allowing greater accuracy in prenatal diagnosis given amounts of fetal material as small as single cells, a major current focus has grown in the development of rapid, cost-effective diagnoses. In Prenatal Diagnosis, top experts provide cutting edge applications for the rapid assessment of fetal aneuploidies and Mendelian disorders on fetal material gained by invasive approaches, as well as procedures being validated for routine, non-invasive clinical analysis of cell free fetal DNA. Following the Methods in Molecular Biology? series format, the chapters feature step-by-step laboratory protocols, lists of the necessary materials, and tips on troubleshooting and avoiding known pitfalls. Thorough and state of the art, Prenatal Diagnosis is an ideal volume for researchers and molecular biologists invested in ever-growing field of prenatal medicine.
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology? series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
Emory Univ., Atlanta, GA. Describes in detail biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions as they occur in living cells. Offers step-by-step laboratory instructions. For researchers. DNLM: Protein Binding--physiology--Laboratory Manuals.
MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting. Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections. Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, MRSA Protocols for Methods in Molecular Biology will make a useful basis for anyone interested in instituting a new method for studying MRSA.