Biowww.net RSS feed http://biowww.net/ Sat, 05 Jul 2008 12:05:20 +0100 FeedCreator 1.7.2 Total RNA and mRNA extraction / isolation principles http://biowww.net/detail-174.html Due to fragility of RNA, extreme caution should be taken to avoid RNA degradation by ribonuclease contamination, high temerature or pH etc. Some good practices of handling RNA when performing total RNA or mRNA extraction: ... biowww Total RNA extraction using TRI REAGENT http://biowww.net/detail-968.html A chemicon tech protocol on isolation total RNA from cell culture or tissues. (Chemicon) ... biowww Poly A+ RNA Isolation from Total RNA via Dynabeads® Oligo (dT)25 http://biowww.net/detail-969.html A pdf protocol on Poly A+ RNA Isolation from total RNA. (TIGR) ... biowww RNA extraction from human mouse cell lines http://biowww.net/detail-974.html This protocol describes the extraction of total RNA from human and mouse cell lines. A pdf protocol developed by Jeremy Hasseman from Institute for Genomic Research. (TIGR) ... biowww RNA extraction from human and animal tissues http://biowww.net/detail-975.html This protocol describes the extraction, purification, and assay of total RNA from human or animal tissue samples. A pdf protocol developed by Jeremy Hasseman from Institute for Genomic Research. (TIGR) ... biowww RNA extraction / RNA isolation guidelines and protocols http://biowww.net/detail-166.html RNA extraction from cells and tissues guideline: - Avoid RNA degradation caused by RNase contamination. - Increase RNA yield by thourough homogenizatio of tissue and cell samples. - Increase RNA quality by proper precipitation of RNA and optimized RNA sto ... biowww