http://biowww.net/ Sat, 19 Jul 2008 00:31:21 +0100 FeedCreator 1.7.2 http://biowww.net/detail-286.html Generally speaking, strategies for DNA extraction and DNA purification depends on your downstream applications, e.g. southern blot hybridization using high molecular weight genomic DNA, DNA fragments for subcloning, or DNA for PCR etc. Some considerations for ... biowww http://biowww.net/detail-614.html Factors affecting quality of DNA purification: 1. DNase: nuclease contamination in DNA preparation is the major concern for high quality DNA purification. So start with fresh tissue and to process tissue samples quickly is the key to lower the risk of DNA d ... biowww http://biowww.net/detail-1097.html This protocol uses phenol/chloroform (1:1) to extract DNA samples. Phenol/chloroform extraction can be repeated for two or more times until protein interface is invisible. Purified DNA can be concentrated by ethanol precipitation. (Alam lab, University of Hawa ... biowww http://biowww.net/detail-635.html Quantification of DNA using the Fluoroimager and Pico Green (Protocol from genome lab at UC Davis). ... biowww http://biowww.net/detail-908.html This protocol is used for YAC DNA extraction. YAC clones are provided as stab cultures in YPD agar. Glycerol stocks of each clone should be made as soon as possible. The clone will remain viable as a stab culture up to one week if stored at 4 c. (Hiroyuki Take ... biowww http://biowww.net/detail-1353.html High molecular weight genomic DNA extraction protocol: Buffer and reagent: Genomic DNA extraction buffer (250ml): 1M Tris.Cl (pH 8.0) 2.5ml 0.5M EDTA (pH 8.0) 50 ml Pancreatic RNase 5 mg 10% SDS ... biowww http://biowww.net/detail-1361.html DNA quantification using spectometry: A general protocol for spectometry quantification of DNA in pdf. ... biowww