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        <title>Biowww.net RSS feed</title>
        <description><![CDATA[BioNews feeds from biowww dot net]]></description>
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        <item>
            <title>FPLC fast performance liquid chromatography protocol</title>
            <link>http://biowww.net/detail-537.html</link>
            <description>The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. 

 ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Protocol for Packing a Reversed-Phase Microcapillary Column</title>
            <link>http://biowww.net/detail-551.html</link>
            <description>By Hookeun Lee, Proteomecenter.org. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>MBP-fusion protein chromatography purification protocol version 2.0</title>
            <link>http://biowww.net/detail-1225.html</link>
            <description>A very detailed protocol of using chromatography and FPLC to purify MBP-fusion protein in pdf file written by Ken Johnson, Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisco ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>The Chrom-Ed Chromatography Series</title>
            <link>http://biowww.net/detail-1275.html</link>
            <description>An online chromatography book edited by Dr. R. P. W. Scott.

Content:

Principles and Practice of Chromatography 
Introduction 
The Development Process 
Chromatography Nomenclature 
Factors Controlling Retention 
Factors Affecting the Magnitude ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>High Performance Liquid Chromatography HPLC: A Users Guide</title>
            <link>http://biowww.net/detail-1165.html</link>
            <description>A user guide on HPLC from Analytical Spectroscopy Research Group, Advanced Science and Technology Center at University of Kentucky.

Content:

About this Guide

Applications

Bibliography

History

Manufacturers

Technical Assistance 

T ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>metall ion affinity purification of proteins</title>
            <link>http://biowww.net/detail-85.html</link>
            <description>are there other (competitive/better) methods for purifying proteins 
 using affinity to chelated metal ions than his6 / nickel? ... ...
 
 ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>source for simple chromatography columns</title>
            <link>http://biowww.net/detail-136.html</link>
            <description>Could anyone please point me to a cheap source for plasic (poly ethylene or similar) columns suitable for 1 to 5 ml of charged agarose? ... ...
 
 ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>HPLC Trouble Shooting</title>
            <link>http://biowww.net/detail-161.html</link>
            <description>HPLC Trouble Shooting guide from Thomson Instrument Company.
 
 Abnormal Peak Shape 
 Fluctuation in Elution time 
 Fluctuation in Baseline 
 Noise in Baseline 
 Rise in Pressure
 ... ...
 
 URL:  ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>HPLC Interactive troubleshooting guide from Varian</title>
            <link>http://biowww.net/detail-447.html</link>
            <description>Varian inc. Troubleshooting include:

Problems with Baselines
Problems with Clogged Tubing
Problems with Leaks
Problems with Peaks
Problems with Pressure 

Column Use &amp;Care Data
Column Washing
Sorbent Specifications ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>An interactive troubleshooting guide on High Performance Liquid Chromatography</title>
            <link>http://biowww.net/detail-449.html</link>
            <description>Some covered problems:

 1.Peaks 
Broad peaks
Ghost peaks
Negative peaks
Peak doubling
Peak fronting
Peak tailing
Spikes
 2.Leaks 
Leak at column or fittings
Leak at detector
Leak at injection valve
Leak at pump
3.Recovery 
Poor sample recovery ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Downloadable HPLC Troubleshooting</title>
            <link>http://biowww.net/detail-450.html</link>
            <description>From MACHEREY-NAGEL Inc.

Content:

chapter 1
broad peaks 
fronting 
tailing 
 
chapter 2
double peaks 
negative peaks 
spikes 
ghost peaks 
 
chapter 3
lack of sensitivity 
poor sample recovery 
high back pressure 
 
 ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>LC Troubleshooting guide</title>
            <link>http://biowww.net/detail-451.html</link>
            <description>From VWR international Ltd. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>HPLC troubleshooting guide from Waters</title>
            <link>http://biowww.net/detail-454.html</link>
            <description>This is a 68 pages PDF troubleshooting brochure from Waters. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>HPLC Troubleshooting Guide from Supelco.</title>
            <link>http://biowww.net/detail-455.html</link>
            <description>20 pages PDF troubleshooting guide from Supleco, Sigma-Aldrich. ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Chromatography Equations</title>
            <link>http://biowww.net/detail-452.html</link>
            <description>Some useful general, GC, TLC equations. (from VMR interanational) ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>pKa of Common acids and Common Bases</title>
            <link>http://biowww.net/detail-453.html</link>
            <description>pKa of Common acids and Common Bases (from VMR international). ...</description>
            <author>biowww</author>
        </item>
        <item>
            <title>Free webcast of HPLC Troubleshooting from PE</title>
            <link>http://biowww.net/detail-448.html</link>
            <description>Webcast supplied by PE. You will have to register to access the webcasting content.

HPLC Troubleshooting (Part 2 of 2*): Recognizing diagnostic clues and interpreting chromatographic results

Duration: 117 minutes ...</description>
            <author>biowww</author>
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