http://biowww.net/ Fri, 16 May 2008 01:30:25 +0100 FeedCreator 1.7.2 http://biowww.net/detail-362.html This is an introduction of 2D-gel from Wellcome Trust. ... biowww http://biowww.net/detail-363.html A very detailed protocol on preparing 2D electrophoresis and many useful tips. Protocol is in pdf format ... biowww http://biowww.net/detail-214.html Some buffer recipes and running conditions for protein electrophoresis from invitrogen.(link changed, last modified on 6/11/05) (Invitrogen) ... biowww http://biowww.net/detail-260.html This is a buffer recipe for making SDS-Polyacrylamide (SDS-PAGE) gels. It uses 40% polyacrylamide solution to cast 6%, 7.5%, 8%, 10% and 12% SDS-PAGE gels for SDS-PAGE electrophoresis. ( Breeden lab, Fred Hutchinson Cancer Research Center) ... biowww http://biowww.net/detail-711.html GeBAflex-tube gel extraction kit is based on a single-use tube style, single step for rapid extraction of Protein, RNA, DNA, and complexes of RNA-proteins, DNA-protein and Protein-Protein from any gel. (GeBA) ... biowww http://biowww.net/detail-713.html Pierce protocol on protein purification from polyacrylamide gels. (Pierece) ... biowww http://biowww.net/detail-815.html A brief polyacrylamide gel drying procedure from Finkbeiner lab, UCSF. ... biowww http://biowww.net/detail-844.html A thesis (pdf) by Lars Pedersen describes and proposes solutions to some of the currently most important problems in pattern recognition and image analysis of two-dimensional gel electrophoresis (2DGE) images. (Lars Pedersen, Technical University of Denmark) ... biowww http://biowww.net/detail-1178.html An PVDF membrane protein staining protocol from NCBA, China. ... biowww http://biowww.net/detail-477.html Tutorial written by Dr James R. Jefferies, Parasitology Group, Institute of Biological Sciences, University of Wales at Aberystwyth, UK. ... biowww http://biowww.net/detail-1341.html A tutorial by Dr James R. Jefferies ( Parasitology Group, Institute of Biological Sciences, University of Wales at Aberystwyth). Content: 1. What is 2D Gel Electrophoresis? 2. What is it used for? 3. How is it Performed? 4. How is t ... biowww http://biowww.net/detail-1220.html Nice introduction on native or non-denaturing gel electrophoresis with gel images. (Alliance Protein Laboratories Inc.) "Native" or "non-denaturing" gel electrophoresis is run in the absence of SDS. While in SDS-PAGE the electrophoretic mobility of proteins ... biowww http://biowww.net/detail-1415.html Protein electrophoresis overview 1. SDS-PAGE electrophoresis: use to determine the molecular weight of proteins. Measuring Molecular Weight with SDS-PAGE (LogMW versus the relat ... biowww http://biowww.net/detail-802.html The "Hall of Shame" presents examples of some of the worst gels students have run in past labs. They represent many of the ways one can mess up a gel (but not all of them - we're still finding new ways!). See what features of your own gel(s) were unsatisfactor ... biowww http://biowww.net/detail-56.html We occasionally need to resolve hyper- and under-phosphorylated forms of a protein (pRb) on SDS-PAGE gels for Western blot analysis. Individuals familiar with these isoforms will know that they differ by an apparent (maximum of) 6 Kd, and separation i ... biowww http://biowww.net/detail-58.html Coomassie Blue Staining of SDS-PAGE Gel Protocol for coomassie blue staining of polyacrylamide gels: Coomassie Blue Staining of SDS-PAGE Gel ... biowww http://biowww.net/detail-74.html How can I make my own gel dryer for plyacrylamide gels? Already have vacuum pump and oven. ... ... ... biowww http://biowww.net/detail-76.html A while ago I've seen a post that referenced a paper about the subject. The claim was it is a lot cheaper and works better than Tris/Glycine and on a par with CAPS-based solutions. We do awful lot of Westerns and although the most expensive part of ... biowww http://biowww.net/detail-112.html I have a simple question, but it's hard for me. I run SDS-PAGE using the protein samples prepared by myself, and stain the gel with commassie blue after ecletrophoresis. The troublesome thing is that there is always smear on the gel according to the p ... biowww http://biowww.net/detail-117.html I have some protein samples that were electroeluted from a SDS-polyacrylamide gel, so they are with SDS and the usual components of the gel and the running buffer. I want to remove all these components as I want to perform a digestion of thes ... biowww http://biowww.net/detail-134.html Does anyone know how to calculate for how long an SDS-PAGE electrophoresis buffer can be re-used. I remember a talk at a conference where the speaker calculated for how long a electrophoresis buffer could be used before exhaustion. Any ... ... ... biowww http://biowww.net/detail-465.html University of Pennsylvania, Genomics institute, Proteomics core facility guidline on 2DE sample preparation. ... biowww http://biowww.net/detail-1181.html This troubleshooting guide details common problems observed when performing gel electrophoresis and suggests ways in which they can be avoided. (Dr. Tony Dixon, the University of Leeds) Content: 1. No distinct spots are visible 2. Individual pro ... biowww http://biowww.net/detail-1204.html Some tips (buffers and gel characteristics etc) and troubleshooting guide on protein gel electrophoresis by Les Lane. ... biowww http://biowww.net/detail-1170.html SUMOplot™ can help you to explain larger MWs than expected on SDS gels due to attachment of SUMO protein (11kDa) at multiple positions of your protein. (Abgent Inc.) SUMO-1 (small ubiquitin-related modifier; also known as PIC1, UBL1, Sentrin, GMP1, an ... biowww http://biowww.net/detail-1167.html The rising interest in proteomics has catapulted 2D gel electrophoresis to the forefront of modern research techniques. It can be used to analyze global protein expression patterns or used to examine the differential expression of proteins in comparable sample ... biowww