http://biowww.net/ Thu, 03 Jul 2008 22:25:09 +0100 FeedCreator 1.7.2 http://biowww.net/detail-881.html This is the Bart's cookbook on immunoprecipitaiton. It gives some general principles on performing immunoprecipitation using different lysis buffers.(Sefton Lab, Salk Institute). Content: RIPA buffer NP40 buffer PBS TN Boiling in SDS Antisera ... biowww http://biowww.net/detail-384.html Immunoprecipitation is a technique that permits the purification of specific proteins for which a antibody has been raised. This primary antibody is either already bound to agarose or can be bound to the protein A/agarose beads during the procedure in order to ... biowww http://biowww.net/detail-609.html This protocol uses total cell extracts to analyze putative protein-protein interactions in eukaryotic cells. One can also use nuclear extracts as a source for this protocol (Morris lab at University of Pennsylvania). ... biowww http://biowww.net/detail-652.html Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is routinely used in a variety of applications: to determine the molecular weights of protein antigens, to study pro ... biowww http://biowww.net/detail-50.html Selection guide for phosphatase inhibitors cocktails (Merck). Phosphatase inhibitor cocktails is used to prevent dephosphorylation of proteins in protein biochemistry applications. Commonly used protein phosphatase inhibitors protect dephosphorylation of se ... biowww http://biowww.net/detail-53.html I am using Protein A Sepharose (Pharmacia) for immune precipitations. I elute immune complexes after binding with laemmli buffer (SDS-PAGE loading buffer). Is there anyone who has experience, whether this very expensive material can be regenerated ... biowww http://biowww.net/detail-81.html We are trying to do a "simple" immunoprecipitation in which the unbound fraction will be assayed for RNA processing activity. We find that protein A-agarose beads have little non-specific RNase activity. After adding serum (immune or non-immune), we w ... biowww http://biowww.net/detail-149.html I am trying to assess the degree of phosphorylation a receptor undergoes in response to it's ligand plus various other factors. If I apply the ligand to my cells and then extract in the presence of sodium orthovanadate and do a western using an antibo ... biowww http://biowww.net/detail-208.html In an immunoprecipitation-western blot I used rabbit polyclonal antibody and protein A agarose beads(protein A is covalently bind to beads) to pull down antigen complex. In following western blot I used rabbit polyclonal antibody as primary antibody and HRP-an ... biowww http://biowww.net/detail-209.html General troubleshooting tips of immunoprecipitation, western blot and immunohistochemistry from Chemicon. Link: troubleshooting tips of IP, western and immunostaining This link was lasted ... biowww http://biowww.net/detail-650.html A brief troubleshooting guide on immunoprecipitation - kinase assay. (Upstate) Determine the optimal stimulation time and temperature for each individual kinase that is to be tested. This is essential since optimal stimulation periods are often short and ca ... biowww http://biowww.net/detail-651.html Troubleshooting guide from Sigma. It addresses immunoprecipitation problems on: 1. No binding 2. High background or unwanted precipitates ... biowww http://biowww.net/detail-679.html What is a good starting concentration of each protein to have? What volume? I know that my proteins are tight-binding, so dissociation should not be a big problem. (post from NWFSC forum). ... biowww http://biowww.net/detail-774.html A Immunoprecipitation troubleshooting guide from Stressgen Biotechnologies. ... biowww http://biowww.net/detail-1063.html A troubleshooting guide on Immunoaffinity purification (Immunoprecipitation) procedure from Roche. ... biowww http://biowww.net/detail-1138.html A troubleshooting guide on immunoprecipitation. (Agrisera) Problems: No binding High background signal ... biowww