Biowww.net RSS feed http://biowww.net/ Mon, 06 Oct 2008 03:27:44 +0100 FeedCreator 1.7.2 Dut/ung in vitro Site-directed Mutagenesis Protocol http://biowww.net/detail-347.html Protocol from Bjorkerman lab at CIT. Originally written by M. Davis and P. Bjorkman. ... biowww Some commercial kits and their brief introduction (biocompare) http://biowww.net/detail-350.html Several commercially available site-directed mutagenesis kits and their brief introduction re given in biocompare.com web site. ... biowww Site-directed Mutagenesis using PCR http://biowww.net/detail-351.html From: Molecular Biology: Current Innovations and Future Trends. Eds. A.M. Griffin and H.G.Griffin. ISBN 1-898486-01-8 1995 ... biowww QuikChange(tm) Site-Directed Mutagenesis Kit http://biowww.net/detail-352.html Stratagene's QuickChange kit manual. ... biowww DpnI mediated site-directed Mutagenesis http://biowww.net/detail-353.html Protocol from Nonet lab at Washington University at St. Louise. A highly effective simple method for making site directed lesions in plasmids without subcloning based on the work of Fisher and Pei (1997). ... biowww General protocol on construction of mutants, chimeras and truncated recombinant plasmid http://biowww.net/detail-1192.html A general overview with some technical details on construction of mutant, chimeras and truncated version of plasmid. (from Liz Greene and Steve Henikoff, Kenesin web site) ... biowww TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening http://biowww.net/detail-912.html This original article is accessible free from NAR. Nucleic Acids Research, 2003, Vol. 31, No. 3 e11 TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening Lei Young* and Qihan Dong Department of Medic ... biowww Site-directed mutagenesis http://biowww.net/detail-22.html I have a cloned gene. I want to mutate a single base-pair. Can anyone recommend any good protocol. Which kit do you suggest? Malay ... biowww Internal deletions by PCR http://biowww.net/detail-142.html I wonder if anybody could tip me on a good and fast way of making internal deletions in a plasmid by PCR. I have previously done it by introducing new restriction sites by site directed mutagenesis (and then digesting and re-ligating) which takes quite a long ... biowww Site directed mutagenesis http://biowww.net/detail-213.html help for site directed mutagenesis. help for site directed mugagenesis ... biowww Mutagenesis for deletions http://biowww.net/detail-217.html Mutagenesis for deletions w/o kit. Source of method ... biowww