http://biowww.net/ Fri, 04 Jul 2008 07:24:51 +0100 FeedCreator 1.7.2 http://biowww.net/detail-939.html Anita¡¯s brief Protocol For Spliting/Plating NIH-3T3 Cells from Meyer lab at Stanfrod University. (PDF file) ... biowww http://biowww.net/detail-954.html A feeder layer tissue culture protocol using 3T3 cells as feeder layer. (Shay/Wrigth Laboratory, SouthWestern Medical Center) ... biowww http://biowww.net/detail-29.html Does anybody know if 293 cells (Human Embryonic Kidney) tolerate serum-free or reduced serum culture medium, and for how long? I'm hoping to purify a protein secreted from a stable 293 line, and the less serum I can get away with, the easier it will b ... biowww http://biowww.net/detail-78.html I am trying to establish stable mammalian cell lines expressing a transfected gene by G418 selection in CHO cells. I treat the transfected cells with G418 @ 400ug/ml (I assume this is this enough ?) but ... ... ... biowww http://biowww.net/detail-103.html I have come round to doing my first stable transfection experiment and have the following questions and wonder if anyone can help: 1) Can anyone provide any reference for the theory of stable transfection? I only know that the DNA goes into the ... biowww