Biowww.net RSS feed http://biowww.net/ Sat, 19 Jul 2008 02:05:56 +0100 FeedCreator 1.7.2 Performing a hunt by interaction mating http://biowww.net/detail-523.html A working protocol in Finley's Lab at Wayne state University school of medicine. (Mikhail G. Kolonin and Russell L. Finley Jr.). Abstract When more than one bait will be used to screen a single library, significant time and resources can be saved ... biowww Examining the function of proteins with the yeast two-hybrid system http://biowww.net/detail-850.html Review and detailed protocol written by Dr. Russ Finley at Finley lab, Wayne State University School of Medicine. The yeast two-hybrid system provides a relatively straight forward approach to understanding protein function. Section II outlines the basic co ... biowww Dual Bait yeast two-hybrid system http://biowww.net/detail-182.html Protocol for construction of dual baid yeast two-hybrid system (Erica Golemis lab at Fox chase cancer center). Protocol content: - Main flowchart of Dual Bait protocol Constructing and Transforming a cI Bait Protein - Choice of cI-fusion vec ... biowww Two Hybrid System TRAFO Protocol http://biowww.net/detail-520.html TRAFO yeast two hybrid protocol for high efficiency yeast transformation. Reference: Agatep, R., R.D. Kirkpatrick, D.L. Parchaliuk, R.A. Woods, and R.D. Gietz (1998) Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrie ... biowww LiOAc yeast transformation http://biowww.net/detail-525.html From Finley's Lab at Wayne state University (This method is based on the procedure of Geitz et al., 1992 Nucleic Acids Research 20:1425). ... biowww Yeast Interaction trap cloning http://biowww.net/detail-524.html A very detailed 1995 technical review on two-hyb system (draft manuscript chaptor in DNA Cloning 2: A Practical Approach, Expression Systems", 2nd Edition, eds D. M. Glover and B. D. Hames. Oxford University Press, 1995). This page is maintained by Finley's La ... biowww Yeast Two-Hybrid Review http://biowww.net/detail-530.html A full accessible online review from Biological Procedures Online (Wim Van Criekinge and Rudi Beyaert, Biol. Proced. Online 1999;2:1-38) Abstract: Genome projects are approaching completion and are saturating sequence databases. This paper discusses ... biowww The Yeast Two-Hybrid Assay Introduction http://biowww.net/detail-531.html A nice illustration and introduction on yeast two-hyb assay (Solmaz Sobhanifar, Bioteach at University of British Columbia, Canada) ... biowww How the two-hybrid system works http://biowww.net/detail-824.html A graphical illustration on how the two-hybrid system works. (Yeast resource center at University of Washington) ... biowww The Gietz Lab Yeast Transformation Home Page http://biowww.net/detail-526.html This site contains information on TRAFO yeast two hyb technique. (Gietz lab at University of Manitoba, Canada) ... biowww Sources of Interaction Trap Reagents http://biowww.net/detail-528.html Page maintained by Erica Golemis' Lab at Fox Chase Cancer Center. Sources of Interaction Trap compatible libraries Sources of Interaction Trap reagents (detailed description) Sources of commercially available reagents ... biowww