http://biowww.net/ Thu, 03 Jul 2008 10:22:57 +0100 FeedCreator 1.7.2 http://biowww.net/detail-264.html This is a standard northern blot hybridization protocol using radioactive labeled probe. The northern blot protocol includes details on total RNA sample preparation, agarose gel electrophoresis using MOPS running buffer, northern blotting membrane transfer pro ... biowww http://biowww.net/detail-101.html I was doing northern analysis with arabidopsis AOS gene. When I used the 5' fragment of the gene as a probe (from the EST), it worked well. However, if I used the 3' fragment as a probe (from PCR), it always hybridized to all the rRNA (including 26S, ... biowww http://biowww.net/detail-194.html We've been having trouble with our RNA degrading during Northern's. We've discovered the culprit is the formaldehyde and particularly the denaturing step (we denatured with formaldehyde in the sample). The pH of our formaldehyde was (4.4) which we had ... biowww