http://biowww.net/ Wed, 06 Aug 2008 16:30:23 +0100 FeedCreator 1.7.2 http://biowww.net/detail-1366.html Targeting HSV amplicon vectors (method) An original technique article on HSV vector construction by Grandi and Wang etc. Targeting HSV amplicon vectors. Methods. 2 ... biowww http://biowww.net/detail-777.html Lentivirus transfection protocol for vector production in 293T cells from Patrick Salmon's Cookbook, department of Microbiology, Geneva School of Medicine, Switzerland. ... biowww http://biowww.net/detail-576.html Clontech protocol (PDF file) on siRNA adenovirus construction. Useful information can be found from CAGT Vector Development Laboratory, Baylor College of Medicine. ... biowww http://biowww.net/detail-812.html Six Well Cell Transfection of 293 Cells Protocol. (Finkbeiner lab, UCSF) This cell transfection protocol uses Lipofectamine plus Reagent for introducing the plasmid DNA into the cell. ... biowww http://biowww.net/detail-955.html A retrovirus infection protocol from Shay/Wrigth Laboratory at Southwestern Medical Center. (MS Doc file). ... biowww http://biowww.net/detail-1222.html Protocol for Plasmid transfection and retrovirus construction in 293 cells. The protocol can be downloaded as word file.(Dr.Gordon Fishell, New York University School of Medicine) ... biowww http://biowww.net/detail-1295.html The protocol describe at below is typical of methods that are used topropagate and purify AAV vectors for experiments both in vitro and in vivo. (Hiroaki Mizukami et al., Jichi Medical School, Japan) ... biowww http://biowww.net/detail-36.html HEK 293 cells are Human Embryonic Kidney cells (HEK cells). 293 cells are generated by transformation of sheared adenovirus 5 genome into human embryonic kidney cells. It is one of the most commonly used cell lines because of its high transfection efficiency. ... biowww http://biowww.net/detail-201.html One of the most frequent laments of using recombinant retroviruses is that the titer does not appear to be sufficient to infect the cells of interest at a high enough frequency to suit your purposes. This is a troubleshooting guide using Phoenix lines. (No ... biowww