http://biowww.net/ Mon, 21 Jul 2008 23:10:48 +0100 FeedCreator 1.7.2 http://biowww.net/detail-787.html A titration protocol for real-time PCR with figures (Dr Patrick SALMON, Geneva School of Medicine). ... biowww http://biowww.net/detail-1305.html Real-time PCR prototocol for genotyping Ts65Dn mice (Don Liu, Cecilia Schmidt, Tim Billings, and Muriel T. Davisson, The Jackson Laboratory). Genotyping Ts65Dn mice is based on doing simultaneous quantitative PCR amplification of a gene or genes in the Ts65 ... biowww http://biowww.net/detail-1306.html Real-time quantitative multiplex RT -PCR (Taqman) protocol. This is performed as a 2-step reaction: 1. cDNA synthesis from DNase I treated total RNA 2. multiplex real-time taqman PCR optimization and PCR. This is a very detailed Taqman real time RT-PCR ... biowww http://biowww.net/detail-1215.html A technical overview on real-time PCR including introduction, primer probe design, data analysis and some recent pdf review articles. (DNA facility, University of Iowa) ... biowww http://biowww.net/detail-1324.html A nice review and collection of technique articles on absolute real-time PCR quantification. (edited by Michael W. Pfaffl) ... biowww http://biowww.net/detail-415.html A introduction of real-time PCR by M.Tevfik Dorak, MD, PhD. It gives background information on real-time PCR, guidlines on designing probe/primer and conditions on performing PCR. ... biowww http://biowww.net/detail-591.html A background introduction on real-time PCR technique (Davidson college, Dept. of Biology). Real-time PCR is able to detect sequence-specific PCR products as they accumulate in "real-time" during the PCR amplification process. As the PCR product of interest ... biowww http://biowww.net/detail-592.html This PDF tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation (Applied biosystems). ... biowww http://biowww.net/detail-1135.html A pdf powerpoint overview on SYBR green real-time PCR. It discussed different methods, data analysis using delta-CT etc (47 pages). (www.gene-quantification.info) SYBR-green real-time is perhaps the best way to quantify gene expression in large scale with h ... biowww http://biowww.net/detail-1136.html Stratagen real-time PCR techbook. It is downloadable as a 50 pages pdf file. Table of Contents: Real-Time vs. Endpoint Quantitative PCR ............................................................................................................... ... biowww http://biowww.net/detail-1330.html List of 10 possible reasons for an unsuccessful real-time PCR (Ambion inc.) More detailed description can be found from this page. Poor primer and probe design. Using poor quality RNA. Not using "master mixes". Introducing cross-contamination. Not u ... biowww http://biowww.net/detail-86.html We are planning to have a new Real Time PCR machine in our lab but still in puzzle to find the best. Can anyone ... ... ... biowww http://biowww.net/detail-111.html I have to do semi-quantitative PCR in order to study the regulation of expression of receptors mRNA. I've already done optimization of annealing temp., MgCl2 conc., etc... If I want to compare the level of two different mRNA in response of a stimul ... biowww http://biowww.net/detail-420.html Real-time PCR protocol with troubleshooting guide (from primersbank) Reference: Xiaowei Wang and Brian Seed (2003) A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Research 31(24): e154; pp.1-8. ... biowww http://biowww.net/detail-417.html This is a public database holding real time PCR primers and probes for popular chemistries (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon) to prevent time-consuming primer design and experimental optimisation. Reference: PATTYN, F., SPELE ... biowww http://biowww.net/detail-419.html PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains about 180,000 primers covering most known human and mouse genes. There are several ways to search f ... biowww http://biowww.net/detail-1168.html Biocompare's qPCR Tutorial presents researchers with an overview of real-time qPCR, identifies the advantages and disadvantages of the various detection technologies, outlines the key issues for optimizing experimental design and offers a brief description of ... biowww