http://biowww.net/ Wed, 03 Sep 2008 08:08:19 +0100 FeedCreator 1.7.2 http://biowww.net/detail-972.html This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, gel electrophoresis, purification, and storage of PCR products. This is a pdf protocol developed by Jeremy Hasseman from Institute for Genomic Research. (TIGR) ... biowww http://biowww.net/detail-72.html I am trying to clearly separate two DNA bands on a gel. The difference between them is 27 bp, and the overall size is between 100 - 200 bp. I have tried 5% acrylamide gels, and get very sharp defined bands, but my negative control lane always looks lo ... biowww http://biowww.net/detail-82.html After PCR with primers containing restriction sites added on, can you immediately digest the PCR fragments prior to purifying by gel extraction? In other words can you avoid an extre gel purification or PCR clean up step prior to restriction digest of ... biowww