http://biowww.net/ Thu, 03 Jul 2008 06:56:51 +0100 FeedCreator 1.7.2 http://biowww.net/detail-178.html The key to LA PCR is an enzyme, TaKaRa LA TaqTM. It is a thermostable DNA polymerase, which possesses 3กฏ to 5กฏ exonuclease activity, or proofreading activity. The efficiency drastically declines when incorrect bases are incorporated. The 3กฏ to 5กฏ exonuclea ... biowww http://biowww.net/detail-308.html From Church lab at Harvard University. Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Foll ... biowww http://biowww.net/detail-1360.html An open access original paper on fidelity of different DNA polymerase used in high fidelity PCR. Nucleic Acids Res. 1996 Sep 15;24(18):3546-51. PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases. Cline J, Braman JC, Hogrefe HH. ... biowww http://biowww.net/detail-89.html I need a protocol for PCR of circular DNA. I would like to amplifie the whole mtDNA (16-17kb) of mytilus by using a long PCR method for circular NA. ... ... ... biowww http://biowww.net/detail-1000.html It describes various problems that may occur during amplification of long DNA sequences and recommendations for avoiding them. Troubleshooting from Roche Applied Bioscience PCR manual. ... biowww