http://biowww.net/ Thu, 03 Jul 2008 09:12:17 +0100 FeedCreator 1.7.2 http://biowww.net/detail-336.html A very detailed protocol from Dept of Medical Genetics, University Hospital Gent, B-9000 Gent Belgium. ... biowww http://biowww.net/detail-337.html There are 50 protocols in this section: Autoradiography for mRNA detection in mouse embryo tissue sections    Protocol Chick, mouse, and Xenopus two colour whole mount in ... biowww http://biowww.net/detail-360.html Protocol for frozen sections with 35S labeled olgonucleotide probe. From GeneDetect.com (PDF version). ... biowww http://biowww.net/detail-332.html This page maintained by Pasteur Institute briefly presents the introduction to fluorescent in situ hybridization technique (FISH). This technique is used for the detection of target molecules with a system of coupled antibodies and fluorochromes. ... biowww http://biowww.net/detail-333.html From Krause Lab Protocols. This procedure is taken from a book chapter. Hughes, S. and Krause, H.M. (1998) Single and double FISH protocols for Drosophila. in: Protocols in confocal microscopy., ed. Stephen Paddock, Humana Press Inc. ... biowww http://biowww.net/detail-335.html A protocol (manual) from www.cancergenetics.com. Fluorescent in situ hybridization (FISH) protocol. ULSTM-dGreen and ULSTM-Rhodamine labeled probes for translocation, locus specific and chromosome copy number detection. ... biowww http://biowww.net/detail-338.html Tech Notes Original Article. Marlyse C. Knuchela et al. Journal of Histochemistry and Cytochemistry, Vol. 48, 285-294, February 2000 Abstract We developed a simple and rapid technique to synthesize single-stranded DNA (ssDNA) probes for fluores ... biowww http://biowww.net/detail-357.html In situ hybridization (ISH) can be used to detect nucleic acid sequences in tissue and cell cultures. Its applications include detection of mRNA expression in situ, chromosomal rearrangement, viral RNA expression in host cell etc. General steps for in situ ... biowww http://biowww.net/detail-361.html These protocols describe non-radioactive methods for in situ hybridization on frozen sections, whole mount embryos and on cultured cells. They have been freely adapted and modified to greater or lesser extents from the protocols of Richard Harland, David Wilki ... biowww http://biowww.net/detail-579.html Cytochemical Detection of mRNA by nonradioactive in situ hybridization A nonradioactive method for in situ hybridization. This page describes the current favorite non-radioactive in situ hybridization t ... biowww http://biowww.net/detail-594.html Protocol by Josiah N. Wilcox, Ph.D., Wilcox lab in Winship Cancer Institute of the School of Medicine of Emory University. ... biowww http://biowww.net/detail-595.html RNA probe preparation protcol for in situ hybridizaiton. by Josiah N. Wilcox, Ph.D. (based on Melton et al., 1984). Wilcox lab at Emory University. ... biowww http://biowww.net/detail-596.html Protocol on tissue section preparation for in situ hybridization. By Dr.Josiah N. Wilcox, Wilcox lab at Emory University. ... biowww http://biowww.net/detail-600.html In situ hybridization protocol on embryo tissue sections. Protocol based on Kornberg et al., Cell 40:45, 1985. ... biowww http://biowww.net/detail-806.html Short protocol on In Situ Hybridization. (Spector lab at Cold Spring Harbor Lab). ... biowww http://biowww.net/detail-904.html This is essentially according to the method recommended by Boehringer Mannheim. Cut 20 ug of plasmid DNA with appropriate enzyme in 100 ul. Pheno1, phenol/chloroform, chloroform and ethanol precipitate. Dissolve in 15 ul ddH20.(Hiroyuki Takeda lab, National in ... biowww http://biowww.net/detail-905.html Protocol by Alex Schier on Wholemount in situ hybridisations on zebrafish embryos using digoxigenin probes. ... biowww http://biowww.net/detail-906.html In this example stain with flh in black (NBT substrate) and with gsc in orange (INT substrate). (Kira, National institute of Genetics, Japan) ... biowww http://biowww.net/detail-216.html I am doing ISHH using 33P labeled-riboprobes on rat brain tissue and I'm having a problem during the development of the slides. I dip each slide in NBT2 emulsion (diluted 1:1 in water), let dry in the dark standing up for 3 hours and then store in sli ... biowww