http://biowww.net/ Thu, 03 Jul 2008 01:53:22 +0100 FeedCreator 1.7.2 http://biowww.net/detail-724.html A detailed protocol on insect cell culture and baculovirus infection. 1.Cells, Media, Growth Characteristics, Freezing and Thawing Cells, and Glassware . 2.Generation of Recombinant Baculovirus. 3.Generation of Recombinant Protein with Baculovirus Infec ... biowww http://biowww.net/detail-344.html Protocol from Bjokerman group at CIT. ... biowww http://biowww.net/detail-716.html Protocol A: Calcium phosphate co-transfection of insect cells Protocol B: Analysis of nonsecreted protein expression Protocol C: Growing and freezing of insect cells Protocols from eBioscience.com ... biowww http://biowww.net/detail-717.html There are many ways for virus to hide, and so this assay is usually an underestimate of the actual number of virus present. For instance, a pfu (plaque forming unit) may not find a cell by the time the attachment period is ended or it may stick to the plate wh ... biowww http://biowww.net/detail-718.html Sf9 cells can be frozen in a manner quite similar to methods used to preserve mammalian cells. This involves putting them in a suitable medium, including 7.5-10% DMSO, and a controlled freezing rate. (Dr. Jim Litts, baculovirus.com) ... biowww http://biowww.net/detail-719.html Infection of suspension cultures is the most common way of producing protein in the baculovirus system. Suspension cultures are easily scaleable from about 10 mL to more that 100 L. Generally, the suspension cultures are maintained in serum free media. These m ... biowww http://biowww.net/detail-720.html Infection of suspension cultures is the most common way of producing protein in the baculovirus system. Suspension cultures are easily scaleable from about 10 mL to more that 100 L. Generally, the suspension cultures are maintained in serum free media. These m ... biowww http://biowww.net/detail-721.html If your recombinant baculovirus stocks have been lost or have "gone off", virus can usually be recovered from frozen pellets of baculovirus-infected insect cells. Virus in these pellets is stable for many years, especially if stored at -70 C or less. (Robin Cl ... biowww http://biowww.net/detail-723.html A mini review by Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma state University. In a previous session, advantages of expressing heterologous genes in E. coli were discussed. While high levels of expression in bacterial systems are common, prob ... biowww http://biowww.net/detail-722.html Amplifying low titer stock can be a little tricky. The most important determinant of success is the general vigor of the particular construct that you have. Some virus constructs replicate very efficiently, and they're easy. Others are not so cooperative. (Dr. ... biowww