http://biowww.net/ Sun, 05 Oct 2008 13:29:33 +0100 FeedCreator 1.7.2 http://biowww.net/detail-962.html This protocol is from Machida lab at Japan National Institute of Bioscience & Human-Technology and is based on Based on Bio-Techniques,23, 300-303 (1997). ... biowww http://biowww.net/detail-964.html A free accessible original paper on PCR footprinting. Reference: Nucleic Acids Res. 1994 Feb 11;22(3):532-3 A modified protocol for in vivo footprinting by ligation-mediated polymerase chain reaction. Dimitrova D, Giacca M, Falaschi A. I ... biowww http://biowww.net/detail-1035.html A short protocol on DNase I protection from Molecular Biology of Nuclear Receptors, France. You will have first to determine the concentration of DNaseI that cleaves 30-50% of your input DNA. A rule of thumb is to multiply this amount by 10 to get the adeq ... biowww http://biowww.net/detail-1036.html Detailed protocols for genetic footprinting, a technique for high-resolution mapping of the functional organization of cloned genes (Singh, Crowley and Brown (1997) PNAS 94: 1304-1309). Reference: Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1304-9. Hig ... biowww http://biowww.net/detail-961.html Footprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. This brief introduction is from Kimball's Biology Pages. This page also contains a nice illustration on principle of footprinting. ... biowww