Biowww.net RSS feed http://biowww.net/ Wed, 27 Aug 2008 04:08:31 +0100 FeedCreator 1.7.2 Protocol For Stable Transfection in RBL Cells http://biowww.net/detail-936.html A brief protocol for stable transfection in RBL cells from Meyer Lab at Stanford University. (PDF file) ... biowww Protocol for stable transfection of Cl-8 or S2 cells http://biowww.net/detail-942.html A protocol modified from Qiagen's SuperFect Transfection Reagent Handbook. Protocol from Tabas lab, Columbia University. (MS Doc file) ... biowww Lentivirus-delivered stable gene silencing by RNAi in primary cells http://biowww.net/detail-1207.html An open accessible original article on construction lentivirus to knock down gene in primary cells. Reference: Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA. 2003 Apr;9(4):493-501. Stewart SA, Dykxhoorn DM, Pallis ... biowww Stable transfection http://biowww.net/detail-1392.html Stable transfection is used to introduce recombinant DNA into eukaryotic cells through integration into host cells's genome. As opposed to transient transfection, stable transfection can retain the expression of foreign DNA. ... biowww