Biowww.net RSS feed http://biowww.net/ Mon, 21 Jul 2008 20:11:29 +0100 FeedCreator 1.7.2 3' RACE PCR http://biowww.net/detail-329.html 3' RACE PCR Protocol: 1. First strand cDNA synthesis: - Reaction set up (total volume 20 ul) cDNA synthesis buffer 4 ul dNTP mixture 2 ul oligo-dT anchor primer 1 ul total RNA ... biowww 5' RACE PCR http://biowww.net/detail-126.html 5' RACE PCR Protocol 1. First strand cDNA synthesis: - Reaction set up: cDNA synthesis buffer 4 ul dNTP mixture 2 ul gene specific primer 1 1 ul total RNA ... biowww RACE amplification protocol http://biowww.net/detail-903.html A detailed protocol on performing 5' and 3' RACE PCR. (Hiroyuki Takeda lab, National institute of Genetics). ... biowww cDNA Synthesis with Oligo dT Primers: First strand synthesis http://biowww.net/detail-1040.html A pdf protocol from Lahn lab at University of Chicago. This may be done with total RNA or poly-A RNA prepped from cell. Here one copy of cDNA will be made with a poly-T primer. (May isolate total RNA and then prep mRNA by selection with oligoT beads to bi ... biowww