http://biowww.net/ Tue, 05 Aug 2008 09:30:26 +0100 FeedCreator 1.7.2 http://biowww.net/detail-1002.html The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T7 and T3, and ... biowww http://biowww.net/detail-1004.html Protocol of RNase protection assay for quantitating RNA levels from either polyA+ selected RNA or crude RNA preps. (Mike A. Dyer, Harvard University) ... biowww http://biowww.net/detail-1007.html This method can be used to detect and quantitate mRNA, to map mRNA termini and to determine the position of introns within the corresponding gene. (Gerard R. Lazo, USDA) ... biowww http://biowww.net/detail-1008.html A RNase protection assay protocol from J. Kennell at University of Michigan. ... biowww http://biowww.net/detail-1009.html Protocol from the Knight lab at Loyola University. Basic reference: Ausubel,F.M. et al. 1989.Current Protocols in Molecular Biology. John Wiley and sons, N.Y. Templates are cloned into pGEM vectors (Promega) that contain the bacterial RNA polymerase pro ... biowww http://biowww.net/detail-1003.html A promega technote written by Steven Ekenberg and Geoff Hudson. The Promega RNase Protection Assay System provides the reagents necessary to map RNAs moreaccurately than obtained with Northern blot procedures. In addition, the system uses RNase ONE(TM) whi ... biowww http://biowww.net/detail-1005.html A technical bulletin article from Ambion on linearity of RNase protection assay. (Ambion). The amount of protected probe is directly proportional to the amount of target mRNA in the sample. If an internal control probe or synthetic sense strand is included ... biowww http://biowww.net/detail-1006.html A technical bulletin article on Choice of Ribonucleases for a Ribonuclease Protection Assay. (Ambion inc). Ribonuclease protection assays (RPAs) are a technique used for detection and quantitation of specific RNAs. The method is based on the ability of ribo ... biowww http://biowww.net/detail-1010.html A open access journal mini review on ribonuclease protection assay. (J. B. Rottman, Millennium Pharmaceuticals) The ribonuclease protection assay is a sensitive and accurate method to measure mRNA expression. The major advantages of the assay are that multi ... biowww